Decreased susceptibility of K. lactis mutants impaired in the function of cytochrome c, cytochrome c1 and cytochrome-c oxidase to fluconazole, bifonazole and amphotericin B in comparison with the isogenic wild-type strain was observed. Flow cytometry with rhodamine 6G did not show any changes in the accumulation of the dye in the mutant cells compared with the corresponding wild-type strain. Sterol analysis showed similar overall amount of sterols in both wild-type and mutant cells. Taking into account the increased amphotericin B resistance and significantly diminished susceptibility of mutant cells to lyticase digestion, the cell wall structure and/or composition may probably be responsible for the observed changes in the susceptibility of mutants to the antifungal compounds used.

• MeSH
• Amphotericin B pharmacology   MeSH
• Antifungal Agents pharmacology   MeSH
• Cell Wall chemistry   drug effects   MeSH
• Glucan Endo-1,3-beta-D-Glucosidase metabolism   MeSH
• Drug Resistance, Fungal * MeSH
• Fungal Proteins genetics   metabolism   MeSH
• Kluyveromyces cytology   drug effects   genetics   physiology   MeSH
• Microbial Sensitivity Tests MeSH
• Mitochondria drug effects   genetics   physiology   MeSH
• Multienzyme Complexes metabolism   MeSH
• Mutation * MeSH
• Blotting, Northern MeSH
• Peptide Hydrolases metabolism   MeSH
• Flow Cytometry MeSH
• Sterols metabolism   MeSH
• Electron Transport drug effects   genetics   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Amphotericin B MeSH
• Antifungal Agents MeSH
• Glucan Endo-1,3-beta-D-Glucosidase MeSH
• Fungal Proteins MeSH
• lyticase MeSH Browser
• Multienzyme Complexes MeSH
• Peptide Hydrolases MeSH
• Sterols MeSH