The objective of this study was to perform molecular surveillance for assessing the spread of carbapenemase-producing Pseudomonas aeruginosa in Czech hospitals. One hundred thirty-six carbapenemase-producing isolates were recovered from 22 hospitals located throughout the country. Sequence type 357 (ST357) dominated (n = 120) among carbapenemase producers. One hundred seventeen isolates produced IMP-type (IMP-7 [n = 116] and IMP-1 [n = 1]) metallo-β-lactamases (MβLs), 15 produced the VIM-2 MβL, and the remaining isolates expressed the GES-5 enzyme. The blaIMP-like genes were located in three main integron types, with In-p110-like being the most prevalent (n = 115). The two other IMP-encoding integrons (In1392 and In1393) have not been described previously. blaVIM-2-carrying integrons included In59-like, In56, and a novel element (In1391). blaGES-5 was carried by In717. Sequencing data showed that In-p110-like was associated with a Tn4380-like transposon inserted in genomic island LESGI-3 in the P. aeruginosa chromosome. The other integrons were also integrated into the P. aeruginosa chromosome. These findings indicated the clonal spread of ST357 P. aeruginosa, carrying the IMP-7-encoding integron In-p110, in Czech hospitals. Additionally, the sporadic emergence of P. aeruginosa producing different carbapenemase types, associated with divergent or novel integrons, punctuated the ongoing evolution of these bacteria.

• Klíčová slova
• GES, Illumina sequencing, ST111, ST235, VIM, class 1 integrons, genomic islands (GIs), integrative conjugative element (ICE),
• MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální chromozomy chemie   MeSH
• beta-laktamasy genetika   metabolismus   MeSH
• epidemiologické monitorování MeSH
• exprese genu MeSH
• genomové ostrovy MeSH
• genotyp MeSH
• incidence MeSH
• integrony MeSH
• izoenzymy genetika   metabolismus   MeSH
• karbapenemy farmakologie   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• mnohočetná bakteriální léková rezistence genetika   MeSH
• multilokusová sekvenční typizace MeSH
• nemocnice MeSH
• pseudomonádové infekce epidemiologie   mikrobiologie   MeSH
• Pseudomonas aeruginosa účinky léků   enzymologie   genetika   izolace a purifikace   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• multicentrická studie MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH
• Názvy látek
• antibakteriální látky MeSH
• beta-lactamase IMP-7 protein, Pseudomonas aeruginosa MeSH Prohlížeč
• beta-laktamasy MeSH
• izoenzymy MeSH
• karbapenemy MeSH