Antibodies have different avidities that can be evaluated using modified enzyme-linked immunosorbent assay (ELISA) techniques. We determined levels and avidities of antibodies to light (NFL) and medium (NFM) subunits of neurofilaments and tau protein in serum and cerebrospinal fluid (CSF) from 26 patients and anti-tau antibody levels and their avidities in 20 multiple sclerosis (MS) patients and 20 age- and sex-matched controls. Each sample was analyzed using both standard ELISA and also using a similar ELISA protocol with the addition of urea. The avidities of anti-neurocytoskeletal antibodies were higher in the CSF than those in serum (anti-NFL, p < 0.0001; anti-tau, p < 0.01; anti-NFM, n.s.). There was no relationship between avidities in serum and CSF for individual anti-neurocytoskeletal antibodies. We did not observe the relationship among the avidities of various anti-neurocytoskeletal antibodies. The avidities of anti-tau antibodies in the CSF were significantly higher in the MS patients than those in the controls (p < 0.0001). The study demonstrates the differences in avidities of CSF or serum neurocytoskeletal antibodies measured as the urea resistance by ELISA method. Avidity determination of anti-neurocytoskeletal antibodies could contribute to the evaluation of the immunological status of patients.

• MeSH
• afinita protilátek MeSH
• dospělí MeSH
• imunoglobulin G krev   mozkomíšní mok   imunologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• neurofilamentové proteiny imunologie   MeSH
• proteiny tau imunologie   MeSH
• protilátky anti-idiotypické krev   mozkomíšní mok   imunologie   MeSH
• roztroušená skleróza krev   mozkomíšní mok   imunologie   MeSH
• senioři MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• imunoglobulin G MeSH
• neurofilament protein L MeSH Prohlížeč
• neurofilament protein M MeSH Prohlížeč
• neurofilamentové proteiny MeSH
• proteiny tau MeSH
• protilátky anti-idiotypické MeSH

BACKGROUND: Antibodies against tau protein indicate an interaction between the immune system and the neurocytoskeleton and therefore may reflect axonal injury in multiple sclerosis (MS). METHODOLOGY/PRINCIPAL FINDINGS: The levels and avidities of anti-tau IgG antibodies were measured using ELISA in paired cerebrospinal fluid (CSF) and serum samples obtained from 49 MS patients and 47 controls. Anti-tau antibodies were significantly elevated intrathecally (p<0.0001) in the MS group. The CSF anti-tau antibody levels were lower in MS patients receiving therapy than those without treatment (p<0.05). The avidities of anti-tau antibodies were higher in the CSF than in the serum (MS group p<0.0001; controls p<0.005). Anti-tau avidities in the CSF were elevated in MS patients in comparison with controls (p<0.05), but not in serum. CONCLUSIONS: MS patients have higher levels of intrathecal anti-tau antibodies. Anti-tau antibodies have different avidities in different compartments with the highest values in the CSF of MS patients.

• MeSH
• afinita protilátek imunologie   MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mícha imunologie   patologie   MeSH
• proteiny tau imunologie   MeSH
• protilátky anti-idiotypické krev   mozkomíšní mok   MeSH
• roztroušená skleróza krev   mozkomíšní mok   imunologie   terapie   MeSH
• studie případů a kontrol MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• proteiny tau MeSH
• protilátky anti-idiotypické MeSH