The paper presents a prospective cohort study. Observed group was formed of children with plaque psoriasis (n=19) treated by Goeckerman therapy (GT). The study describes adverse (side) effects associated with application of GT (combined exposure of 3% crude coal tar ointment and UV radiation). After GT we found significantly increased markers of oxidative stress (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanosine, and 8-hydroxyguanine), significantly increased levels of benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) DNA adducts (BPDE-DNA), and significantly increased levels of total number of chromosomal aberrations in peripheral lymphocytes. We found significant relationship between (1) time of UV exposure and total number of aberrated cells and (2) daily topical application of 3% crude coal tar ointment (% of body surface) and level of BPDE-DNA adducts. The findings indicated increased hazard of oxidative stress and genotoxic effects related to the treatment. However, it must be noted that the oxidized guanine species and BPDE-DNA adducts also reflect individual variations in metabolic enzyme activity (different extent of bioactivation of benzo[a]pyrene to BPDE) and overall efficiency of DNA/RNA repair system. The study confirmed good effectiveness of the GT (significantly decreased PASI score).

• MeSH
• 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide analysis   chemistry   MeSH
• DNA Adducts analysis   chemistry   MeSH
• Benzo(a)pyrene chemistry   toxicity   MeSH
• Chromosome Aberrations * MeSH
• Coal Tar pharmacology   therapeutic use   MeSH
• Child MeSH
• DNA chemistry   MeSH
• Keratolytic Agents pharmacology   therapeutic use   MeSH
• Cohort Studies MeSH
• Humans MeSH
• Lymphocytes cytology   metabolism   MeSH
• Ointments pharmacology   therapeutic use   MeSH
• Adolescent MeSH
• Oxidative Stress drug effects   MeSH
• DNA Damage drug effects   MeSH
• Child, Preschool MeSH
• Prospective Studies MeSH
• Psoriasis drug therapy   metabolism   pathology   MeSH
• Ultraviolet Rays * MeSH
• Check Tag
• Child MeSH
• Humans MeSH
• Adolescent MeSH
• Male MeSH
• Child, Preschool MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide MeSH
• DNA Adducts MeSH
• benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA MeSH Browser
• Benzo(a)pyrene MeSH
• Coal Tar MeSH
• DNA MeSH
• Keratolytic Agents MeSH
• Ointments MeSH

Some specific antibodies indicate the presence of antigenic structures on DNA (DNA adducts) that can play an important role in the process of mutagenesis and/or carcinogenesis. They indicate the presence of increased genotoxic potential (hazard) prior to the formation of disease (primary prevention). The present study was focused on the serum level of benzo[a]pyrene 7,8-diol-9,10-epoxide-DNA adducts antibodies (anti-BPDE-DNA) in psoriatic patients (n = 55) dermally exposed to different levels of polycyclic aromatic hydrocarbons (PAHs). The general goal of the study was to contribute to better understanding of the value of the assumed biomarker (anti-BPDE-DNA) for evaluation of the organism's answer to genotoxic exposure to PAHs. Elevated level of exposure to PAHs resulted in the increased level of anti-BPDE-DNA. However, almost all levels of anti-BPDE-DNA ranged within the field of low values. Both variants of GT (CCT-3% and CCT-5%) induced higher expression of anti-BPDE-DNA in the group of nonsmokers. Significant relations between the level of anti-BPDE-DNA and PASI score, total duration of the therapy, or time of UVR exposure were not found. Further studies are needed to reduce interpretation uncertainty of this promising bioindicator.

• MeSH
• 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide MeSH
• DNA Adducts blood   immunology   MeSH
• Biomarkers blood   MeSH
• Coal Tar therapeutic use   MeSH
• Adult MeSH
• Smoking blood   MeSH
• Skin drug effects   immunology   MeSH
• Middle Aged MeSH
• Humans MeSH
• Adolescent MeSH
• Polycyclic Aromatic Hydrocarbons toxicity   MeSH
• Antibodies blood   MeSH
• Psoriasis blood   immunology   pathology   therapy   MeSH
• Aged MeSH
• Ultraviolet Therapy MeSH
• Check Tag
• Adult MeSH
• Middle Aged MeSH
• Humans MeSH
• Adolescent MeSH
• Male MeSH
• Aged MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide MeSH
• DNA Adducts MeSH
• benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA MeSH Browser
• Biomarkers MeSH
• Coal Tar MeSH
• Polycyclic Aromatic Hydrocarbons MeSH
• Antibodies MeSH