With the increasing demand for noninvasive approaches in monitoring head and neck cancer, circulating nucleic acids have been shown to be a promising tool. We focused on the global transcriptome of serum samples of head and neck squamous cell carcinoma (HNSCC) patients in comparison with healthy individuals. We compared gene expression patterns of 36 samples. Twenty-four participants including 16 HNSCC patients (from 12 patients we obtained blood samples 1 year posttreatment) and 8 control subjects were recruited. The Illumina HumanWG-6 v3 Expression BeadChip was used to profile and identify the differences in serum mRNA transcriptomes. We found 159 genes to be significantly changed (Storey's P value <0.05) between normal and cancer serum specimens regardless of factors including p53 and B-cell lymphoma family members (Bcl-2, Bcl-XL). In contrast, there was no difference in gene expression between samples obtained before and after surgery in cancer patients. We suggest that microarray analysis of serum cRNA in patients with HNSCC should be suitable for refinement of early stage diagnosis of disease that can be important for development of new personalized strategies in diagnosis and treatment of tumours but is not suitable for monitoring further development of disease.

• MeSH
• analýza hlavních komponent MeSH
• apoptóza genetika   MeSH
• demografie MeSH
• dlaždicobuněčné karcinomy hlavy a krku MeSH
• dospělí MeSH
• genom lidský genetika   MeSH
• lidé středního věku MeSH
• lidé MeSH
• messenger RNA krev   genetika   MeSH
• mikročipová analýza * MeSH
• nádorový supresorový protein p53 metabolismus   MeSH
• nádory hlavy a krku krev   genetika   patologie   MeSH
• regulace genové exprese u nádorů MeSH
• senioři MeSH
• signální transdukce genetika   MeSH
• spinocelulární karcinom krev   genetika   patologie   MeSH
• stanovení celkové genové exprese MeSH
• studie případů a kontrol MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• messenger RNA MeSH
• nádorový supresorový protein p53 MeSH