The inhibition effect of the selected heavy metals (Ag+, Cd2+, Cu2+, and Hg2+) on glucose oxidase (GOx) enzyme from Aspergillus niger (EC 1.1.3.4.) was studied using a new amperometric biosensor with an electrochemical transducer based on a glassy carbon electrode (GCE) covered with a thin layer of multi-wall carbon nanotubes (MWCNTs) incorporated with ruthenium(IV) oxide as a redox mediator. Direct adsorption of multi-wall carbon nanotubes (MWCNTs) and subsequent covering with Nafion® layer was used for immobilization of GOx. The analytical figures of merit of the developed glucose (Glc) biosensor are sufficient for determination of Glc in body fluids in clinical analysis. From all tested heavy metals, mercury(II) has the highest inhibition effect. However, it is necessary to remember that cadmium and silver ions also significantly inhibit the catalytic activity of GOx. Therefore, the development of GOx biosensors for selective indirect determination of each heavy metal still represents a challenge in the field of bioelectroanalysis. It can be concluded that amperometric biosensors, differing in the utilized enzyme, could find their application in the toxicity studies of various poisons.

• Klíčová slova
• amperometric biosensor, glucose oxidase, heavy metals, non-competitive inhibition,
• MeSH
• Aspergillus niger enzymologie   MeSH
• biosenzitivní techniky * MeSH
• elektrochemické techniky přístrojové vybavení   metody   MeSH
• elektrody MeSH
• glukosa analýza   MeSH
• glukosaoxidasa antagonisté a inhibitory   metabolismus   MeSH
• inhibitory enzymů farmakologie   toxicita   MeSH
• kalibrace MeSH
• limita detekce MeSH
• nanotrubičky uhlíkové MeSH
• peroxid vodíku analýza   MeSH
• sloučeniny ruthenia chemie   MeSH
• těžké kovy farmakologie   toxicita   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• glukosa MeSH
• glukosaoxidasa MeSH
• inhibitory enzymů MeSH
• nanotrubičky uhlíkové MeSH
• peroxid vodíku MeSH
• ruthenium tetraoxide MeSH Prohlížeč
• sloučeniny ruthenia MeSH
• těžké kovy MeSH