The review covering the development of capillary electrophoresis with capacitively coupled contactless conductivity detection from 2020 to 2024 is the latest in a series going back to 2004. The article considers applications employing conventional capillaries and planar lab-on-chip devices as well as fundamental and technical developments of the detector and complete electrophoresis instrumentation.

• Klíčová slova
• capacitively coupled contactless conductivity detection, capillary electrophoresis, microchip electrophoresis, review,
• MeSH
• design vybavení MeSH
• elektrická vodivost MeSH
• elektroforéza kapilární * přístrojové vybavení   metody   MeSH
• laboratoř na čipu MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

Capillary electrophoresis (CE) with contactless conductivity detection (C4D) represents a strong tool for determining amino acids in clinical samples. This chapter provides detailed instructions for CE/C4D determination of the branched-chain amino acids (BCAAs) valine, isoleucine, and leucine in human plasma, which can be readily employed in physiological studies. Baseline separation of all the BCAAs is achieved on a short separation length equal to 18 cm in optimized background electrolyte consisting of 3.2 M acetic acid dissolved in 20% v/v methanol with addition of 1.0% v/v INST-coating solution. The analysis time does not exceed 3 min and the limit of detection is 0.4 μM for all BCAAs. The pretreatment of human plasma is very simple and is based on fourfold plasma dilution by acetonitrile and subsequent filtration. Only 50 μL of plasma is used for the analysis. The high sensitivity of the CE/C4D method is achieved by injecting a large volume of sample, combined with application of negative pressure to flush the acetonitrile zone out of the capillary.

• Klíčová slova
• Branched-chain amino acids, Capillary electrophoresis, Contactless conductivity detection, Human plasma, Large-volume sample stacking, Pressure-assisted analysis, Rapid determination,
• MeSH
• elektrická vodivost * MeSH
• elektroforéza kapilární metody   MeSH
• fyziologie * MeSH
• kalibrace MeSH
• lidé MeSH
• referenční standardy MeSH
• větvené aminokyseliny krev   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• větvené aminokyseliny MeSH