The natural flavonoid quercetin is known to activate the transcription factor Nrf2, which regulates the expression of cytoprotective enzymes such as heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase 1 (NQO1). In this study, a novel semisynthetic flavonoid 7-O-galloylquercetin (or quercetin-7-gallate, 3) was prepared by direct galloylation of quercetin, and its effect on the Nrf2 pathway was examined. A luciferase reporter assay showed that 7-O-galloylquercetin, like quercetin, significantly activated transcription via the antioxidant response element in a stably transfected human AREc32 reporter cell line. In addition, 7-O-galloylquercetin caused the accumulation of Nrf2 and induced the expression of HO-1 at both the mRNA and protein levels in murine macrophage RAW264.7 cells. The induction of HO-1 by 7-O-galloylquercetin was significantly suppressed by N-acetyl-l-cysteine and SB203580, indicating the involvement of reactive oxygen species and p38 mitogen-activated protein kinase activity, respectively. HPLC/MS analyses also showed that 7-O-galloylquercetin was not degalloylated to quercetin, but it was conjugated with glucuronic acid and/or methylated in RAW264.7 cells. Furthermore, 7-O-galloylquercetin was found to increase the protein levels of Nrf2 and HO-1, and also the activity of NQO1 in murine hepatoma Hepa1c1c7 cells. Taken together, we conclude that 7-O-galloylquercetin increases Nrf2 activity and induces Nrf2-dependent gene expression in RAW264.7 and Hepa1c1c7 cells.

• Klíčová slova
• Heme oxygenase-1, Metabolism, Methyl gallate, Nrf2, Quercetin, Quercetin-7-gallate,
• MeSH
• biotransformace účinky léků   MeSH
• enzymová indukce účinky léků   MeSH
• faktor 2 související s NF-E2 metabolismus   MeSH
• hemoxygenasa-1 biosyntéza   MeSH
• hmotnostní spektrometrie MeSH
• inhibitory proteinkinas farmakologie   MeSH
• lidé MeSH
• metabolom účinky léků   MeSH
• mitogenem aktivované proteinkinasy p38 metabolismus   MeSH
• myši MeSH
• NAD(P)H dehydrogenasa (chinon) metabolismus   MeSH
• nádorové buněčné linie MeSH
• quercetin chemická syntéza   chemie   farmakologie   MeSH
• RAW 264.7 buňky MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• regulace genové exprese účinky léků   MeSH
• reportérové geny MeSH
• viabilita buněk účinky léků   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• faktor 2 související s NF-E2 MeSH
• hemoxygenasa-1 MeSH
• inhibitory proteinkinas MeSH
• mitogenem aktivované proteinkinasy p38 MeSH
• NAD(P)H dehydrogenasa (chinon) MeSH
• quercetin MeSH
• reaktivní formy kyslíku MeSH