Nejvíce citovaný článek - PubMed ID 24125695
Characterization of pbt genes conferring increased Pb2+ and Cd2+ tolerance upon Achromobacter xylosoxidans A8
Although stinging nettle (Urtica dioica) has been shown to reduce HM (heavy metal) content in soil, its wider phytoremediation potential has been neglected. Urtica dioica was cultivated in soils contaminated with HMs or polychlorinated biphenyls (PCBs). After four months, up to 33% of the less chlorinated biphenyls and 8% of HMs (Zn, Pb, Cd) had been removed. Bacteria were isolated from the plant tissue, with the endophytic bacteria Bacillus shackletonii and Streptomyces badius shown to have the most significant effect. These bacteria demonstrated not only benefits for plant growth, but also extreme tolerance to As, Zn and Pb. Despite these results, the native phytoremediation potential of nettles could be improved by biotechnologies. Transient expression was used to investigate the functionality of the most common constitutive promoter, CaMV 35S in Urtica dioica. This showed the expression of the CUP and bphC transgenes. Collectively, our findings suggest that remediation by stinging nettle could have a much wider range of applications than previously thought.
- MeSH
- biodegradace * MeSH
- genetické inženýrství metody MeSH
- geneticky modifikované rostliny genetika metabolismus MeSH
- kadmium metabolismus MeSH
- látky znečišťující půdu metabolismus MeSH
- olovo metabolismus MeSH
- polychlorované bifenyly analýza metabolismus MeSH
- promotorové oblasti (genetika) * genetika MeSH
- půda chemie MeSH
- regulace genové exprese u rostlin genetika MeSH
- těžké kovy analýza metabolismus MeSH
- Urtica dioica genetika metabolismus MeSH
- zinek metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kadmium MeSH
- látky znečišťující půdu MeSH
- olovo MeSH
- polychlorované bifenyly MeSH
- půda MeSH
- těžké kovy MeSH
- zinek MeSH