BACKGROUND: In view of the ever-increasing representation of Staphylococcus spp. strains resistant to various antibiotics, the development of in vivo models for evaluation of novel antimicrobials is of utmost importance. METHODS: In this article, we describe the development of a fully immunocompetent porcine model of extensive skin and soft tissue damage suitable for testing topical antimicrobial agents that matches the real clinical situation. The model was developed in three consecutive stages with protocols for each stage amended based on the results of the previous one. RESULTS: In the final model, 10 excisions of the skin and underlying soft tissue were created in each pig under general anesthesia, with additional incisions to the fascia performed at the base of the defects and immediately inoculated with Staphylococcus aureus suspension. One pig was not inoculated and used as the negative control. Subsequently, the bandages were changed on Days 4, 8, 11, and 15. At these time points, a filter paper imprint technique (FPIT) was made from each wound for semi-quantitative microbiological evaluation. Tissue samples from the base of the wound together with the adjacent intact tissue of three randomly selected defects of each pig were taken for microbiological, histopathological, and molecular-biological examination. The infection with the inoculated S. aureus strains was sufficient during the whole experiment as confirmed by both FPIT and from tissue samples. The dynamics of the inflammatory markers and clinical signs of infection are also described. CONCLUSIONS: A successfully developed porcine model is suitable for in vivo testing of novel short-acting topical antimicrobial agents.

• Klíčová slova
• Staphylococcus aureus, animal model, antimicrobial agents, porcine model, skin and soft‐tissue infection (SSTI), wound infection,
• MeSH
• antibakteriální látky * aplikace a dávkování   terapeutické užití   farmakologie   MeSH
• aplikace lokální MeSH
• infekce měkkých tkání * farmakoterapie   mikrobiologie   MeSH
• kůže mikrobiologie   patologie   MeSH
• methicilin rezistentní Staphylococcus aureus * účinky léků   MeSH
• modely nemocí na zvířatech * MeSH
• prasata MeSH
• stafylokokové infekce kůže * farmakoterapie   mikrobiologie   MeSH
• stafylokokové infekce * farmakoterapie   mikrobiologie   MeSH
• Staphylococcus aureus * účinky léků   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky * MeSH