Nejvíce citovaný článek - PubMed ID 25123582
Interaction of a novel antimicrobial peptide isolated from the venom of solitary bee Colletes daviesanus with phospholipid vesicles and Escherichia coli cells
We have investigated structural changes of peptides related to antimicrobial peptide Halictine-1 (HAL-1) induced by interaction with various membrane-mimicking models with the aim to identify a mechanism of the peptide mode of action and to find a correlation between changes of primary/secondary structure and biological activity. Modifications in the HAL-1 amino acid sequence at particular positions, causing an increase of amphipathicity (Arg/Lys exchange), restricted mobility (insertion of Pro) and consequent changes in antimicrobial and hemolytic activity, led to different behavior towards model membranes. Secondary structure changes induced by peptide-membrane interaction were studied by circular dichroism, infrared spectroscopy, and fluorescence spectroscopy. The experimental results were complemented by molecular dynamics calculations. An α-helical structure has been found to be necessary but not completely sufficient for the HAL-1 peptides antimicrobial action. The role of alternative conformations (such as β-sheet, PPII or 310-helix) also seems to be important. A mechanism of the peptide mode of action probably involves formation of peptide assemblies (possibly membrane pores), which disrupt bacterial membrane and, consequently, allow membrane penetration.
- Klíčová slova
- antibacterial peptides, circular dichroism, fluorescence, halictine, infrared spectroscopy,
- MeSH
- antibakteriální látky chemie metabolismus MeSH
- fosfatidylcholiny chemie MeSH
- fosfatidylglyceroly chemie MeSH
- hydrofobní a hydrofilní interakce MeSH
- kationické antimikrobiální peptidy chemie metabolismus MeSH
- kinetika MeSH
- konformace proteinů, alfa-helix MeSH
- konformace proteinů, beta-řetězec MeSH
- lipidové dvojvrstvy chemie MeSH
- permeabilita MeSH
- sekvence aminokyselin MeSH
- simulace molekulární dynamiky MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
- fosfatidylcholiny MeSH
- fosfatidylglyceroly MeSH
- kationické antimikrobiální peptidy MeSH
- lipidové dvojvrstvy MeSH
