During homologous recombination, Dbl2 protein is required for localisation of Fbh1, an F-box helicase that efficiently dismantles Rad51-DNA filaments. RNA-seq analysis of dbl2Δ transcriptome showed that the dbl2 deletion results in upregulation of more than 500 loci in Schizosaccharomyces pombe. Compared with the loci with no change in expression, the misregulated loci in dbl2Δ are closer to long terminal and long tandem repeats. Furthermore, the misregulated loci overlap with antisense transcripts, retrotransposons, meiotic genes and genes located in subtelomeric regions. A comparison of the expression profiles revealed that Dbl2 represses the same type of genes as the HIRA histone chaperone complex. Although dbl2 deletion does not alleviate centromeric or telomeric silencing, it suppresses the silencing defect at the outer centromere caused by deletion of hip1 and slm9 genes encoding subunits of the HIRA complex. Moreover, our analyses revealed that cells lacking dbl2 show a slight increase of nucleosomes at transcription start sites and increased levels of methylated histone H3 (H3K9me2) at centromeres, subtelomeres, rDNA regions and long terminal repeats. Finally, we show that other proteins involved in homologous recombination, such as Fbh1, Rad51, Mus81 and Rad54, participate in the same gene repression pathway.

• MeSH
• centromera MeSH
• histonový kód MeSH
• homologní rekombinace * MeSH
• nukleozomy metabolismus   MeSH
• proteiny buněčného cyklu antagonisté a inhibitory   metabolismus   MeSH
• regulace genové exprese u hub * MeSH
• represorové proteiny fyziologie   MeSH
• Schizosaccharomyces pombe - proteiny antagonisté a inhibitory   metabolismus   fyziologie   MeSH
• Schizosaccharomyces genetika   MeSH
• transkripční faktory antagonisté a inhibitory   metabolismus   MeSH
• umlčování genů * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• Dbl2 protein, S pombe MeSH Prohlížeč
• hip1 protein, S pombe MeSH Prohlížeč
• nukleozomy MeSH
• proteiny buněčného cyklu MeSH
• represorové proteiny MeSH
• Schizosaccharomyces pombe - proteiny MeSH
• Slm9 protein, S pombe MeSH Prohlížeč
• transkripční faktory MeSH

Microbial colony growth can serve as a useful readout in assays for studying complex genetic interactions or the effects of chemical compounds. Although computational tools for acquiring quantitative measurements of microbial colonies have been developed, their utility can be compromised by inflexible input image requirements, non-trivial installation procedures, or complicated operation. Here, we present the Spotsizer software tool for automated colony size measurements in images of robotically arrayed microbial colonies. Spotsizer features a convenient graphical user interface (GUI), has both single-image and batch-processing capabilities, and works with multiple input image formats and different colony grid types. We demonstrate how Spotsizer can be used for high-throughput quantitative analysis of fission yeast growth. The user-friendly Spotsizer tool provides rapid, accurate, and robust quantitative analyses of microbial growth in a high-throughput format. Spotsizer is freely available at https://data.csiro.au/dap/landingpage?pid=csiro:15330 under a proprietary CSIRO license.

• Klíčová slova
• bacteria, colony array, large-scale screen, microorganism, synthetic genetic array, yeast,
• MeSH
• algoritmy MeSH
• počítačové zpracování obrazu metody   MeSH
• rychlé screeningové testy metody   MeSH
• Schizosaccharomyces růst a vývoj   MeSH
• software * MeSH
• Publikační typ
• časopisecké články MeSH