Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug. The determination of mephedrone and its metabolites is important for understanding its possible use in medicine. In this work, a method of capillary electrophoresis for the chiral separation of mephedrone and its metabolites was developed. Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates. Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L-1 carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved. For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L-1; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel's fitting test, the linear calibration ranges were determined.

• Klíčová slova
• capillary electrophoresis, chiral separation, cyclodextrin, mephedrone, metabolites,
• MeSH
• biotransformace MeSH
• cyklodextriny chemie   MeSH
• elektroforéza kapilární metody   MeSH
• kalibrace MeSH
• limita detekce MeSH
• methamfetamin analogy a deriváty   analýza   chemie   metabolismus   MeSH
• stereoizomerie MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• cyklodextriny MeSH
• mephedrone MeSH Prohlížeč
• methamfetamin MeSH