BACKGROUND This study was carried out to determine the relationship between the common TMEM-18 (rs4854344, G>T) and NYD-SP18 (rs6971091, G>A) gene variants and weight loss after lifestyle interventions (increased physical activity in conjunction with optimal dietary intake) in overweight/obese children/adolescents. MATERIAL AND METHODS We genotyped 684 unrelated, white, non-diabetic children (age 12.7±2.1 years, average BMI at baseline 30.66±4.80 kg/m²). Anthropometric and biochemical examinations were performed before and after 4 weeks of an intensive lifestyle intervention. RESULTS The mean weight loss achieved was 5.20±2.02 kg (P<0.001). NYDSP-18 AA homozygotes had significantly higher abdominal skinfold value before and after the intervention (both, P=0.001). No significant associations between BMI decrease and the NYD-SP18 and TMEM18 variants were found. Associations between all anthropometrical and biochemical changes and genes remained non-significant after data were adjusted for sex, age, and baseline values. CONCLUSIONS Decreased body weight in overweight/obese children is not significantly influenced by the NYD-SP18 rs6971091 or TMEM18 rs4854344 polymorphisms.

• MeSH
• Adiposity genetics   MeSH
• Exercise MeSH
• Child MeSH
• Genotype MeSH
• Weight Loss genetics   MeSH
• Body Mass Index MeSH
• Nuclear Proteins genetics   metabolism   MeSH
• Polymorphism, Single Nucleotide MeSH
• Humans MeSH
• Membrane Proteins genetics   metabolism   MeSH
• Adolescent MeSH
• Overweight genetics   MeSH
• Obesity genetics   MeSH
• Body Weight genetics   MeSH
• Life Style MeSH
• Check Tag
• Child MeSH
• Humans MeSH
• Adolescent MeSH
• Male MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• FAM71F1 protein, human MeSH Browser
• Nuclear Proteins MeSH
• Membrane Proteins MeSH
• TMEM18 protein, human MeSH Browser

AIM: To replicate the finding that the polymorphism rs6971091 within the NYD-SP18 gene is associated with body mass index (BMI). METHOD: We analysed data of 29,284 adults (46.2% of males, mean age 58.9 (SD 7.3), mean BMI 28.6 (5.0 kg/m2)) examined within the Health Alcohol and Psychosocial Factors in Eastern Europe study in the Czech Republic, Poland, Lithuania and Russia. RESULTS: BMI did not differ by rs6971091 genotype. In men, the mean BMI (SEs) in GG, GA and AA carriers were 27.8 (0.05), 27.9 (0.06) and 27.9 (0.14) kg/m2, respectively, (p = 0.26); in women, the corresponding values were 29.2 (0.06), 29.1 (0.07) and 29.1 (0.16), p = 0.57. In Czech subjects (n = 6,752), for whom the FTO rs17817449 genotype was available, there was no interaction between the NYD-SP18 and FTO polymorphisms in determination of BMI. Adjustment for age, energy and fat intake and physical activity did not materially change the results. There was no association of the NYD-SP18 genotype with waist-hip ratio. CONCLUSION: This study in a large Slavonic population sample suggests that the rs6971091 variant within the NYD-SP18 gene is not an important determinant of obesity in middle-aged persons.

• MeSH
• Alleles MeSH
• Gene Frequency MeSH
• Alpha-Ketoglutarate-Dependent Dioxygenase FTO genetics   metabolism   MeSH
• Genetic Predisposition to Disease MeSH
• Genetic Association Studies MeSH
• Body Mass Index MeSH
• Nuclear Proteins genetics   metabolism   MeSH
• Polymorphism, Single Nucleotide * MeSH
• Cohort Studies MeSH
• Middle Aged MeSH
• Humans MeSH
• Overweight blood   genetics   metabolism   MeSH
• Obesity blood   genetics   metabolism   MeSH
• Waist-Hip Ratio MeSH
• Registries MeSH
• Reproducibility of Results MeSH
• Aged MeSH
• Check Tag
• Middle Aged MeSH
• Humans MeSH
• Male MeSH
• Aged MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Research Support, N.I.H., Extramural MeSH
• Geographicals
• Czech Republic MeSH
• Lithuania MeSH
• Poland MeSH
• Russia MeSH
• Names of Substances
• FAM71F1 protein, human MeSH Browser
• FTO protein, human MeSH Browser
• Alpha-Ketoglutarate-Dependent Dioxygenase FTO MeSH
• Nuclear Proteins MeSH