• Klíčová slova
• Candida, Candida parapsilosis complex, MALDI-TOF, fluorescent capillary electrophoresis, fungi,
• MeSH
• Candida parapsilosis klasifikace   izolace a purifikace   MeSH
• DNA fungální genetika   MeSH
• elektroforéza kapilární metody   normy   MeSH
• fluorescence MeSH
• intergenová DNA genetika   MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• dopisy MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA fungální MeSH
• intergenová DNA MeSH

Molecular techniques in fungal detection and identification represent an efficient complementary diagnostic tool which is increasingly used to overcome limitations of routinely used culture techniques. The aim of this study was to characterize Candida sp. representation in samples from urine, urinary catheter, and ureteral stent biofilm using ITS2 ribosomal DNA (rDNA) amplification followed by fluorescent capillary electrophoresis (f-ITS2-PCR-CE) and to compare the results with those obtained by culture. A total of 419 samples were analyzed, and 106 (25.2%) were found positive, out of which 17 (16%) were polyfungal. The positivity rate did not differ between samples from catheters and stents (23.6% versus 20.9%) or between catheter and stent corresponding urine samples (40.2% versus 30.2%). Ten different Candida species were detected, with Candida parapsilosis (31.4%), Candida albicans (26.5%), and Candida tropicalis (12.4%) predominating. f-ITS2-PCR-CE was evaluated as substantially less time-consuming and 8.3 times more sensitive than the routinely applied culture technique with 1 µl of urine/sonicated fluid inoculated, detecting 67 (19.9%) versus 8 (2.4%) positive samples out of 337 initially analyzed samples. The culture sensitivity considerably improved to 1.7 times lower than that of f-ITS2-PCR-CE after the inoculation volume was increased to 100 µl in the additional 82 samples. Moreover, the molecular technique, unlike routine cultivation, enabled precise pathogen composition determination in polymicrobial samples. In conclusion, the f-ITS2-PCR-CE method was shown to be a quick and efficient tool for culture-independent detection and identification of fungi in urinary tract-related samples, demonstrating a higher sensitivity than culture.

• Klíčová slova
• Candida, biofilms, capillary electrophoresis, fungi, panfungal PCR, polyfungal sample, ureteral stents, urinary catheters,
• MeSH
• biofilmy růst a vývoj   MeSH
• Candida albicans izolace a purifikace   MeSH
• Candida parapsilosis izolace a purifikace   MeSH
• Candida tropicalis izolace a purifikace   MeSH
• Candida klasifikace   izolace a purifikace   MeSH
• diagnostické techniky molekulární přístrojové vybavení   metody   MeSH
• DNA fungální genetika   MeSH
• elektroforéza kapilární metody   MeSH
• fluorescence MeSH
• kandidóza mikrobiologie   moč   MeSH
• lidé MeSH
• močové katétry mikrobiologie   MeSH
• počet mikrobiálních kolonií normy   MeSH
• ribozomální DNA genetika   MeSH
• senioři MeSH
• senzitivita a specificita MeSH
• stenty mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA fungální MeSH
• ribozomální DNA MeSH