The neurohormones arginine-vasopressin (AVP) and oxytocin (OT) synthesised in supraoptic and paraventricular nuclei of neurohypophysis regulate lactation, systemic water homeostasis and nociception. Using transgenic rats expressing AVP and OT tagged with fluorescent proteins we demonstrate that both neurohormones are expressed in sensory neurones both in vitro, in primary cultures, and in situ, in the intact ganglia; this expression was further confirmed with immunocytochemistry. Both neurohormones were expressed in nociceptive neurones immunopositive to transient receptor potential vannilloid 1 (TRPV1) channel antibodies. The AVP and OT-expressing DRG neurones responded to AVP, OT, 50 mM K+ and capsaicin with [Ca2+]i transients; responses to AVP and OT were specifically blocked by the antagonists of V1 AVP and OT receptors. Probing the extracellular incubation saline with ELISA revealed AVP and OT secretion from isolated DRGs; this secretion was inhibited by tetanus toxin (TeNT) indicating the role for vesicular release. Expression of OT, but not AVP in DRG neurones significantly increased during lactation. Together, the results indicate novel physiological roles (possibly related to nociception and mood regulation) of AVP and OT in the sensory neurones.

• MeSH
• dehydratace metabolismus   MeSH
• exocytóza * MeSH
• fluorescence MeSH
• laktace * MeSH
• nervové receptory metabolismus   MeSH
• nocicepce MeSH
• oxytocin metabolismus   MeSH
• potkani transgenní MeSH
• receptory oxytocinu metabolismus   MeSH
• receptory vasopresinů metabolismus   MeSH
• spinální ganglia metabolismus   MeSH
• vasopresiny metabolismus   MeSH
• zadní lalok hypofýzy metabolismus   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• oxytocin MeSH
• receptory oxytocinu MeSH
• receptory vasopresinů MeSH
• vasopresiny MeSH