In recent years interest has grown in the occurrence and the effects of pharmaceuticals in the environment. The aim of this work is to evaluate the risk of fertilizing crops with manure from livestock treated with anthelmintics. The present study was designed to follow the fate of the commonly used anthelmintic drug, ivermectin (IVM) and its metabolites in soybeans (Glycine max (L.) Merr.), a plant that is grown and consumed world-wide for its high content of nutritional and health-beneficial substances. In vitro plantlets and soybean plants, cultivated in a greenhouse, were used for this purpose. Our results showed the uptake of IVM and its translocation to the leaves, but not in the pods and the beans. Four IVM metabolites were detected in the roots, and one in the leaves. IVM exposure decreased slightly the number and weight of the beans and induced changes in the activities of antioxidant enzymes. In addition, the presence of IVM affected the proportion of individual isoflavones and reduced the content of isoflavones aglycones, which might decrease the therapeutic value of soybeans. Fertilization of soybean fields with manure from IVM-treated animals appears to be safe for humans, due to the absence of IVM in beans, the food part of plants. On the other hand, it could negatively affect soybean plants and herbivorous invertebrates.

• Keywords
• anthelmintics, antioxidant enzymes, biotransformation, drug metabolites, isoflavonoids,
• MeSH
• Antioxidants metabolism   MeSH
• Antiparasitic Agents pharmacology   MeSH
• Biological Transport MeSH
• Glycine max drug effects   growth & development   metabolism   MeSH
• Isoflavones metabolism   MeSH
• Ivermectin pharmacology   MeSH
• Plant Roots drug effects   growth & development   metabolism   MeSH
• Plant Leaves drug effects   growth & development   metabolism   MeSH
• Seeds drug effects   growth & development   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Antioxidants MeSH
• Antiparasitic Agents MeSH
• Isoflavones MeSH
• Ivermectin MeSH