Rationale: Loss of histone macroH2A1 induces appearance of cancer stem cells (CSCs)-like cells in hepatocellular carcinoma (HCC). How CSCs interact with the tumor microenvironment and the adaptive immune system is unclear. Methods: We screened aggressive human HCC for macroH2A1 and CD44 CSC marker expression. We also knocked down (KD) macroH2A1 in HCC cells, and performed integrated transcriptomic and secretomic analyses. Results: Human HCC showed low macroH2A1 and high CD44 expression compared to control tissues. MacroH2A1 KD CSC-like cells transferred paracrinally their chemoresistant properties to parental HCC cells. MacroH2A1 KD conditioned media transcriptionally reprogrammed parental HCC cells activated regulatory CD4+/CD25+/FoxP3+ T cells (Tregs). Conclusions: Loss of macroH2A1 in HCC cells drives cancer stem-cell propagation and evasion from immune surveillance.

• Klíčová slova
• adaptive immune system, chemoresistance., hepatocellular carcinoma, histone macroH2A1,
• MeSH
• antigeny CD44 metabolismus   MeSH
• chemorezistence * MeSH
• forkhead transkripční faktory metabolismus   MeSH
• genový knockdown MeSH
• glykolýza MeSH
• hepatocelulární karcinom farmakoterapie   imunologie   metabolismus   patologie   MeSH
• histony metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• metabolomika metody   MeSH
• nádorové buněčné linie MeSH
• nádorové kmenové buňky účinky léků   imunologie   patologie   MeSH
• nádorové mikroprostředí imunologie   MeSH
• nádory jater farmakoterapie   imunologie   metabolismus   patologie   MeSH
• parakrinní signalizace * MeSH
• receptor interleukinu-2 - alfa-podjednotka metabolismus   MeSH
• regulace genové exprese u nádorů MeSH
• regulační T-lymfocyty imunologie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD44 MeSH
• CD44 protein, human MeSH Prohlížeč
• forkhead transkripční faktory MeSH
• FOXP3 protein, human MeSH Prohlížeč
• histony MeSH
• macroH2A histone MeSH Prohlížeč
• receptor interleukinu-2 - alfa-podjednotka MeSH

Cardiovascular disease (CVD), which is the leading cause of death worldwide, is strongly affected by diet. Diet can affect CVD directly by modulating the composition of vascular plaques, and indirectly by affecting the rate of aging. This review summarizes research on the relationships of fasting, meal timing, and meal frequency with CVD incidence and progression. Relevant basic research studies, epidemiological studies, and clinical studies are highlighted. In particular, we discuss both intermittent and periodic fasting interventions with the potential to prevent and treat CVD.

• Klíčová slova
• Blood pressure, Caloric restriction, Cardiovascular disease, Fasting, Fasting mimicking diet,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH