The aim of our study was to determine the PPARγ agonism and hypoglycemic activity of natural phenolics isolated from Paulownia tomentosa and Morus alba. We started with a molecular docking preselection, followed by in vitro cell culture assays, such as PPARγ luciferase reporter gene assay and PPARγ protein expression by Western blot analysis. The ability of the selected compounds to induce GLUT4 translocation in cell culture and lower blood glucose levels in chicken embryos was also determined. Among the thirty-six plant phenolic compounds, moracin M showed the highest hypoglycemic effect in an in ovo experiment (7.33 ± 2.37%), followed by mulberrofuran Y (3.84 ± 1.34%) and diplacone (3.69 ± 1.37%). Neither moracin M nor mulberrofuran Y showed a clear effect on the enhancement of GLUT4 translocation or agonism on PPARγ, while diplacone succeeded in both (3.62 ± 0.16% and 2.4-fold ± 0.2, respectively). Thus, we believe that the compounds moracin M, mulberrofuran Y, and diplacone are suitable for further experiments to elucidate their mechanisms of action.

• Klíčová slova
• PPARγ, diabetes mellitus, hypoglycemic, natural products, plant phenolics,
• MeSH
• fenoly * chemie   farmakologie   izolace a purifikace   MeSH
• hypoglykemika * chemie   farmakologie   izolace a purifikace   MeSH
• kuřecí embryo MeSH
• lidé MeSH
• Morus * chemie   MeSH
• myši MeSH
• PPAR gama * agonisté   metabolismus   genetika   chemie   MeSH
• přenašeč glukosy typ 4 metabolismus   genetika   MeSH
• rostlinné extrakty * chemie   farmakologie   izolace a purifikace   MeSH
• simulace molekulového dockingu MeSH
• zvířata MeSH
• Check Tag
• kuřecí embryo MeSH
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• fenoly * MeSH
• hypoglykemika * MeSH
• PPAR gama * MeSH
• přenašeč glukosy typ 4 MeSH
• rostlinné extrakty * MeSH