Parenteral nutrition (PN) is often associated with the deterioration of liver functions (PNALD). Omega-3 polyunsaturated fatty acids (PUFA) were reported to alleviate PNALD but the underlying mechanisms have not been fully unraveled yet. Using omics´ approach, we determined serum and liver lipidome, liver proteome, and liver bile acid profile as well as markers of inflammation and oxidative stress in rats administered either ω-6 PUFA based lipid emulsion (Intralipid) or ω-6/ω-3 PUFA blend (Intralipid/Omegaven) via the enteral or parenteral route. In general, we found that enteral administration of both lipid emulsions has less impact on the liver than the parenteral route. Compared with parenterally administered Intralipid, PN administration of ω-3 PUFA was associated with 1. increased content of eicosapentaenoic (EPA)- and docosahexaenoic (DHA) acids-containing lipid species; 2. higher abundance of CYP4A isoenzymes capable of bioactive lipid synthesis and the increased content of their potential products (oxidized EPA and DHA); 3. downregulation of enzymes involved CYP450 drug metabolism what may represent an adaptive mechanism counteracting the potential negative effects (enhanced ROS production) of PUFA metabolism; 4. normalized anti-oxidative capacity and 5. physiological BAs spectrum. All these findings may contribute to the explanation of ω-3 PUFA protective effects in the context of PN.

• MeSH
• Emulsions MeSH
• Enteral Nutrition methods   MeSH
• Phospholipids MeSH
• Liver metabolism   MeSH
• Rats MeSH
• Eicosapentaenoic Acid chemistry   MeSH
• Docosahexaenoic Acids chemistry   MeSH
• Oxygen metabolism   MeSH
• Lipidomics MeSH
• Lipids chemistry   MeSH
• Malondialdehyde metabolism   MeSH
• Metabolomics MeSH
• Fatty Acids, Unsaturated metabolism   MeSH
• Fatty Acids, Omega-3 chemistry   MeSH
• Oxidative Stress MeSH
• Parenteral Nutrition methods   MeSH
• Rats, Wistar MeSH
• Proteome metabolism   MeSH
• Reactive Oxygen Species metabolism   MeSH
• Fish Oils MeSH
• Soybean Oil MeSH
• Inflammation MeSH
• Bile Acids and Salts analysis   MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Male MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Comparative Study MeSH
• Names of Substances
• Emulsions MeSH
• Phospholipids MeSH
• Eicosapentaenoic Acid MeSH
• Docosahexaenoic Acids MeSH
• Oxygen MeSH
• Lipids MeSH
• Malondialdehyde MeSH
• Fatty Acids, Unsaturated MeSH
• Fatty Acids, Omega-3 MeSH
• Proteome MeSH
• Reactive Oxygen Species MeSH
• Fish Oils MeSH
• Soybean Oil MeSH
• soybean oil, phospholipid emulsion MeSH Browser
• Bile Acids and Salts MeSH