For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-antibody and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.

• MeSH
• Electrophoresis, Capillary MeSH
• Fluorescence MeSH
• Mass Spectrometry * MeSH
• Immunoassay methods   MeSH
• Immunoglobulin G analysis   MeSH
• Quantum Dots chemistry   MeSH
• Laser Therapy * MeSH
• Molecular Imprinting * MeSH
• Mice MeSH
• Signal Processing, Computer-Assisted MeSH
• Polymers chemistry   MeSH
• Proteins analysis   MeSH
• Cadmium Compounds chemistry   MeSH
• Sulfides chemistry   MeSH
• Animals MeSH
• Check Tag
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• cadmium sulfide MeSH Browser
• Immunoglobulin G MeSH
• Polymers MeSH
• Proteins MeSH
• Cadmium Compounds MeSH
• Sulfides MeSH