Immunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological complex samples. Current methods of labelling antibodies with elemental labels followed by detection by inductively coupled plasma mass spectrometry (ICP-MS) allow detection of multiple antigens in parallel in a single analysis. Using the laser ablation (LA) modality (LA-ICP-MS), it is also possible to monitor the spatial distribution of biogenic elements. Moreover, the employment of metal nanoparticle-labeled antibodies expands the applicability also to molecular imaging by LA-ICP-MS. In this work, conjugates of model monoclonal antibody (DO-1, recognizing p53 protein) with various metal nanoparticles-based labels were created and utilized in dot-blot analysis in order to compare their benefits and disadvantages. Based on experiments with the p53 protein standard, commercial kits of gold nanoparticles proved to be the most suitable for the preparation of conjugates. The LA-ICP-MS demonstrated very good repeatability, wide linear dynamic range (0.1-14 ng), and limit of detection was calculated as a 1.3 pg of p53 protein.

• Klíčová slova
• antibody, dot-blot, protein p53,
• MeSH
• europium chemie   MeSH
• hmotnostní spektrometrie MeSH
• imunoblotting MeSH
• kadmium chemie   MeSH
• kovové nanočástice chemie   MeSH
• kvantové tečky chemie   MeSH
• lasery MeSH
• lidé MeSH
• limita detekce MeSH
• monoklonální protilátky chemie   farmakologie   MeSH
• nádorový supresorový protein p53 antagonisté a inhibitory   MeSH
• stříbro chemie   MeSH
• zlato chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• europium MeSH
• kadmium MeSH
• monoklonální protilátky MeSH
• nádorový supresorový protein p53 MeSH
• stříbro MeSH
• TP53 protein, human MeSH Prohlížeč
• zlato MeSH