Gasoline engine emissions have been classified as possibly carcinogenic to humans and represent a significant health risk. In this study, we used MucilAir™, a three-dimensional (3D) model of the human airway, and BEAS-2B, cells originating from the human bronchial epithelium, grown at the air-liquid interface to assess the toxicity of ordinary gasoline exhaust produced by a direct injection spark ignition engine. The transepithelial electrical resistance (TEER), production of mucin, and lactate dehydrogenase (LDH) and adenylate kinase (AK) activities were analyzed after one day and five days of exposure. The induction of double-stranded DNA breaks was measured by the detection of histone H2AX phosphorylation. Next-generation sequencing was used to analyze the modulation of expression of the relevant 370 genes. The exposure to gasoline emissions affected the integrity, as well as LDH and AK leakage in the 3D model, particularly after longer exposure periods. Mucin production was mostly decreased with the exception of longer BEAS-2B treatment, for which a significant increase was detected. DNA damage was detected after five days of exposure in the 3D model, but not in BEAS-2B cells. The expression of CYP1A1 and GSTA3 was modulated in MucilAir™ tissues after 5 days of treatment. In BEAS-2B cells, the expression of 39 mRNAs was affected after short exposure, most of them were upregulated. The five days of exposure modulated the expression of 11 genes in this cell line. In conclusion, the ordinary gasoline emissions induced a toxic response in MucilAir™. In BEAS-2B cells, the biological response was less pronounced, mostly limited to gene expression changes.

• Keywords
• MucilAir™, air-liquid interface, bronchial epithelial cells, gasoline emissions, toxicity,
• MeSH
• Adenylate Kinase metabolism   MeSH
• Bronchi cytology   MeSH
• DNA Breaks, Double-Stranded MeSH
• Electric Impedance MeSH
• Epithelial Cells drug effects   metabolism   MeSH
• Cells, Cultured MeSH
• L-Lactate Dehydrogenase metabolism   MeSH
• Humans MeSH
• Mucins metabolism   MeSH
• Toxicity Tests methods   MeSH
• Transcriptome MeSH
• Vehicle Emissions toxicity   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Adenylate Kinase MeSH
• L-Lactate Dehydrogenase MeSH
• Mucins MeSH
• Vehicle Emissions MeSH

The biological effects induced by complete engine emissions in a 3D model of the human airway (MucilAirTM) and in human bronchial epithelial cells (BEAS-2B) grown at the air-liquid interface were compared. The cells were exposed for one or five days to emissions generated by a Euro 5 direct injection spark ignition engine. The general condition of the cells was assessed by the measurement of transepithelial electrical resistance and mucin production. The cytotoxic effects were evaluated by adenylate kinase (AK) and lactate dehydrogenase (LDH) activity. Phosphorylation of histone H2AX was used to detect double-stranded DNA breaks. The expression of the selected 370 relevant genes was analyzed using next-generation sequencing. The exposure had minimal effects on integrity and AK leakage in both cell models. LDH activity and mucin production in BEAS-2B cells significantly increased after longer exposures; DNA breaks were also detected. The exposure affected CYP1A1 and HSPA5 expression in MucilAirTM. There were no effects of this kind observed in BEAS-2B cells; in this system gene expression was rather affected by the time of treatment. The type of cell model was the most important factor modulating gene expression. In summary, the biological effects of complete emissions exposure were weak. In the specific conditions used in this study, the effects observed in BEAS-2B cells were induced by the exposure protocol rather than by emissions and thus this cell line seems to be less suitable for analyses of longer treatment than the 3D model.

• Keywords
• 3D models, cell monocultures, complete engine emissions, gene expression,
• MeSH
• Biomarkers MeSH
• Models, Biological * MeSH
• Endoplasmic Reticulum Chaperone BiP MeSH
• Electric Impedance MeSH
• Epithelial Cells drug effects   metabolism   MeSH
• Gene Expression MeSH
• Humans MeSH
• Mucins biosynthesis   MeSH
• Respiratory Mucosa drug effects   metabolism   MeSH
• Vehicle Emissions toxicity   MeSH
• Environmental Exposure adverse effects   MeSH
• DNA Breaks MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Biomarkers MeSH
• Endoplasmic Reticulum Chaperone BiP MeSH
• HSPA5 protein, human MeSH Browser
• Mucins MeSH
• Vehicle Emissions MeSH