Bacillus subtilis 115 grew in a medium with amino acids and glucose with the maximum specific growth rates mu of 1.20-1.10/h in the temperature range of 45-48 degrees C. Activity of the extracellular neutral proteinase excreted by 1.3 mg/mL dry mass during 8 h of the postexponential and stationary growth phases decreased from its maximum value of 0.23 TU/mL at 40 degrees C to 0.13 and 0.06 TU/mL at 45 and 48 degrees C, respectively. Formation of the extracellular serine proteinase decreased even more - from 0.18 TU/mL at 40 degrees C to 0.06 and 0.03 TU/mL at 45 and 48 degrees C, respectively. Sporulation, expressed as the portion of sporangia with refractile spores at the 6th h of the stationary phase decreased from 46% at 40 degrees C to 17 and 3% at 45 and 48 degrees C, respectively.

• MeSH
• Bacillus subtilis physiology   MeSH
• Cell Division MeSH
• Endopeptidases biosynthesis   MeSH
• Metalloendopeptidases biosynthesis   MeSH
• Spores, Bacterial physiology   MeSH
• Subtilisins biosynthesis   MeSH
• Hot Temperature MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Comparative Study MeSH
• Names of Substances
• Endopeptidases MeSH
• Metalloendopeptidases MeSH
• Subtilisins MeSH

Growth, differential rate of alpha-amylase synthesis and production characteristics of Bacillus subtilis DP 1 (isolate from starch materials) in comparison with 10 Bacillus strains were examined in batch fermentation. The effect of the carbon and nitrogen source was evaluated with regard to cell growth and enzyme production. The pH optimum of enzyme activity was 6.5 and temperature optimum of 60 degrees C.

• MeSH
• alpha-Amylases biosynthesis   MeSH
• Bacillus subtilis enzymology   growth & development   MeSH
• Cell Division physiology   MeSH
• Nitrogen physiology   MeSH
• Kinetics MeSH
• Hydrogen-Ion Concentration MeSH
• Culture Media pharmacology   MeSH
• Temperature MeSH
• Carbon physiology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• alpha-Amylases MeSH
• Nitrogen MeSH
• Culture Media MeSH
• Carbon MeSH