Analytical limitations considerably hinder our understanding of the impacts of the physicochemical properties of nanomaterials (NMs) on their biological fate in organisms. Here, using a fit-for-purpose analytical workflow, including dosing and emerging analytical techniques, NMs present in organisms are characterized and quantified across an aquatic food chain. The size and shape of gold (Au)-NMs are shown to control the number of Au-NMs attached to algae that were exposed to an equal initial concentration of 2.9 × 1011 particles mL-1. The Au-NMs undergo size/shape-dependent dissolution and agglomeration in the gut of the daphnids, which determines the size distribution of the NMs accumulated in fish. The biodistribution of NMs in fish tissues (intestine, liver, gills, and brain) also depends on NM size and shape, although the highest particle numbers per unit of mass are almost always present in the fish brain. The findings emphasize the importance of physicochemical properties of metallic NMs in their biotransformations and tropic transfers.

• MeSH
• Bioaccumulation MeSH
• Biotransformation MeSH
• Water Pollutants, Chemical chemistry   metabolism   MeSH
• Daphnia metabolism   MeSH
• Species Specificity MeSH
• Microalgae metabolism   MeSH
• Nanostructures * chemistry   MeSH
• Food Chain * MeSH
• Fishes metabolism   MeSH
• Tissue Distribution MeSH
• Particle Size MeSH
• Gold chemistry   metabolism   MeSH
• Animals MeSH
• Check Tag
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Water Pollutants, Chemical MeSH
• Gold MeSH