The nuclear lamina is a dense network of intermediate filaments beneath the inner nuclear membrane. Composed of A-type lamins (lamin A/C) and B-type lamins (lamins B1 and B2), the nuclear lamina provides a scaffold for the nuclear envelope and chromatin, thereby maintaining the structural integrity of the nucleus. A-type lamins are also found inside the nucleus where they interact with chromatin and participate in gene regulation. Viruses replicating in the cell nucleus have to overcome the nuclear envelope during the initial phase of infection and during the nuclear egress of viral progeny. Here, we focused on the role of lamins in the replication cycle of a dsDNA virus, mouse polyomavirus. We detected accumulation of the major capsid protein VP1 at the nuclear periphery, defects in nuclear lamina staining and different lamin A/C phosphorylation patterns in the late phase of mouse polyomavirus infection, but the nuclear envelope remained intact. An absence of lamin A/C did not affect the formation of replication complexes but did slow virus propagation. Based on our findings, we propose that the nuclear lamina is a scaffold for replication complex formation and that lamin A/C has a crucial role in the early phases of infection with mouse polyomavirus.

• Keywords
• VP1, lamin A/C, lamin B, mouse polyomavirus, viral replication centres,
• MeSH
• Cell Nucleus metabolism   virology   MeSH
• Phosphorylation MeSH
• Tumor Virus Infections virology   pathology   metabolism   genetics   MeSH
• Nuclear Lamina * metabolism   virology   MeSH
• Nuclear Envelope metabolism   virology   MeSH
• Lamin Type A * metabolism   genetics   MeSH
• Lamin Type B metabolism   genetics   MeSH
• Mice MeSH
• Polyomavirus Infections * virology   metabolism   genetics   pathology   MeSH
• Polyomavirus * genetics   pathogenicity   physiology   MeSH
• Virus Replication * MeSH
• Capsid Proteins metabolism   genetics   MeSH
• Animals MeSH
• Check Tag
• Mice MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Lamin Type A * MeSH
• Lamin Type B MeSH
• Capsid Proteins MeSH
• VP1 protein, polyomavirus MeSH Browser