Bone tissue engineering tries to simulate natural behavior of hard tissues. This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp. In situ synthesis resulted in calcium deficient form of HAp with lower crystallinity. The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp. For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001). It seemed to be advantageous improve the properties of the scaffold by in situ synthesizing of HAp directly in the organic matrix.

• Klíčová slova
• biological evaluation, bone tissue engineering, cell viability, hyaluronic acid, hydrogel, hydroxyapatite, in situ synthesis, polyvinyl alcohol, scaffold,
• MeSH
• biokompatibilní materiály chemie   MeSH
• buněčná adheze MeSH
• hemolýza MeSH
• hydrogely chemie   MeSH
• hydroxyapatit chemie   MeSH
• kyselina hyaluronová chemie   MeSH
• lidé MeSH
• osteoblasty cytologie   MeSH
• polyvinylalkohol chemie   MeSH
• proliferace buněk MeSH
• testování materiálů MeSH
• tkáňové inženýrství MeSH
• tkáňové podpůrné struktury chemie   MeSH
• viabilita buněk MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• biokompatibilní materiály MeSH
• hydrogely MeSH
• hydroxyapatit MeSH
• kyselina hyaluronová MeSH
• polyvinylalkohol MeSH

This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing-thawing method. We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, and hemolytic test. The results showed that all types of scaffolds should be safe for use in the human body. The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp. This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold. Based on our results we concluded that the best PVA/HA/HAp combination is in the ratio 3:1:2.

• Klíčová slova
• biological evaluation, bone tissue engineering, hyaluronic acid, hydroxyapatite, polyvinyl alcohol, scaffold,
• MeSH
• biokompatibilní materiály metabolismus   farmakologie   MeSH
• buněčná adheze účinky léků   MeSH
• hydrogely chemie   MeSH
• hydroxyapatit metabolismus   farmakologie   MeSH
• kyselina hyaluronová metabolismus   farmakologie   MeSH
• lidé MeSH
• nádorové buněčné linie MeSH
• osteoblasty metabolismus   MeSH
• polyvinylalkohol metabolismus   farmakologie   MeSH
• proliferace buněk účinky léků   MeSH
• testování materiálů metody   MeSH
• tkáňové inženýrství metody   MeSH
• tkáňové podpůrné struktury chemie   MeSH
• viabilita buněk účinky léků   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• biokompatibilní materiály MeSH
• hydrogely MeSH
• hydroxyapatit MeSH
• kyselina hyaluronová MeSH
• polyvinyl alcohol hydrogel MeSH Prohlížeč
• polyvinylalkohol MeSH