Bone tissue engineering tries to simulate natural behavior of hard tissues. This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp. In situ synthesis resulted in calcium deficient form of HAp with lower crystallinity. The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp. For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001). It seemed to be advantageous improve the properties of the scaffold by in situ synthesizing of HAp directly in the organic matrix.

• Keywords
• biological evaluation, bone tissue engineering, cell viability, hyaluronic acid, hydrogel, hydroxyapatite, in situ synthesis, polyvinyl alcohol, scaffold,
• MeSH
• Biocompatible Materials chemistry   MeSH
• Cell Adhesion MeSH
• Hemolysis MeSH
• Hydrogels chemistry   MeSH
• Durapatite chemistry   MeSH
• Hyaluronic Acid chemistry   MeSH
• Humans MeSH
• Osteoblasts cytology   MeSH
• Polyvinyl Alcohol chemistry   MeSH
• Cell Proliferation MeSH
• Materials Testing MeSH
• Tissue Engineering MeSH
• Tissue Scaffolds chemistry   MeSH
• Cell Survival MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Biocompatible Materials MeSH
• Hydrogels MeSH
• Durapatite MeSH
• Hyaluronic Acid MeSH
• Polyvinyl Alcohol MeSH

This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing-thawing method. We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, and hemolytic test. The results showed that all types of scaffolds should be safe for use in the human body. The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp. This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold. Based on our results we concluded that the best PVA/HA/HAp combination is in the ratio 3:1:2.

• Keywords
• biological evaluation, bone tissue engineering, hyaluronic acid, hydroxyapatite, polyvinyl alcohol, scaffold,
• MeSH
• Biocompatible Materials metabolism   pharmacology   MeSH
• Cell Adhesion drug effects   MeSH
• Hydrogels chemistry   MeSH
• Durapatite metabolism   pharmacology   MeSH
• Hyaluronic Acid metabolism   pharmacology   MeSH
• Humans MeSH
• Cell Line, Tumor MeSH
• Osteoblasts metabolism   MeSH
• Polyvinyl Alcohol metabolism   pharmacology   MeSH
• Cell Proliferation drug effects   MeSH
• Materials Testing methods   MeSH
• Tissue Engineering methods   MeSH
• Tissue Scaffolds chemistry   MeSH
• Cell Survival drug effects   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Biocompatible Materials MeSH
• Hydrogels MeSH
• Durapatite MeSH
• Hyaluronic Acid MeSH
• polyvinyl alcohol hydrogel MeSH Browser
• Polyvinyl Alcohol MeSH