Streptomyces cinnamonensis produces a new substance named AIB (for anti-isobutyrate) factor which, on a solid medium, efficiently counteracts toxic concentrations not only of isobutyrate but also of other salts of short-chain monocarboxylic acids. In the present study we demonstrate that the AIB factor activity is widely spread because this effect was positively detected in 25 of 31 randomly chosen microorganisms (streptomycetes, ascomycetes, zygomycetes and basidiomycetes). The AIB factor produced by the tested microorganisms on an agar media allows for germination, growth, and sporulation of the testing Streptomyces coelicolor on an agar medium containing 20 mmol/L acetate, propionate, butyrate, isobutyrate, valerate, isovalerate, and 2-methylbutyrate. The activity of the AIB factor from different sources towards these substances differs.

• MeSH
• Ascomycota metabolismus   MeSH
• Basidiomycota metabolismus   MeSH
• biologické faktory metabolismus   MeSH
• butyráty antagonisté a inhibitory   MeSH
• druhová specificita MeSH
• hemiterpeny MeSH
• houby metabolismus   MeSH
• isobutyráty MeSH
• kyseliny pentanové antagonisté a inhibitory   MeSH
• Streptomyces metabolismus   MeSH
• substrátová specifita MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• 2-methylbutanoic acid MeSH Prohlížeč
• biologické faktory MeSH
• butyráty MeSH
• hemiterpeny MeSH
• isobutyráty MeSH
• isobutyric acid MeSH Prohlížeč
• isovaleric acid MeSH Prohlížeč
• kyseliny pentanové MeSH
• n-pentanoic acid MeSH Prohlížeč

Mycelium of Streptomyces cinnamonensis mutant strains cultivated in a synthetic medium with glycine produced protoplasts after lysis of cell walls with lysozyme. The protoplast yield was up to 95%. The protoplasts could revert and mycelial forms were thus regenerated. In a sucrose-containing medium the protoplasts stored at 4 degrees C were stable for 2 d.

• MeSH
• glycin MeSH
• kultivační média MeSH
• muramidasa MeSH
• mutace MeSH
• mykologie metody   MeSH
• protoplasty cytologie   fyziologie   MeSH
• Streptomyces cytologie   genetika   fyziologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• glycin MeSH
• kultivační média MeSH
• muramidasa MeSH

Vitamins added to submerged Streptomyces cinnamonensis cultures stimulated the production of monensins. Vitamins B2, B3, B5 and B12 enhanced the production by about 50%, vitamins B1 and B6 by 100%. The addition of biotin in optimal concentration resulted in more than 3-fold increase in total production.

• MeSH
• biotin farmakologie   MeSH
• kultivační média MeSH
• kyselina pantothenová farmakologie   MeSH
• monensin biosyntéza   MeSH
• niacin farmakologie   MeSH
• pyridoxin farmakologie   MeSH
• riboflavin farmakologie   MeSH
• Streptomyces účinky léků   metabolismus   MeSH
• thiamin farmakologie   MeSH
• vitamin B 12 farmakologie   MeSH
• vitamin B komplex farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• biotin MeSH
• kultivační média MeSH
• kyselina pantothenová MeSH
• monensin MeSH
• niacin MeSH
• pyridoxin MeSH
• riboflavin MeSH
• thiamin MeSH
• vitamin B 12 MeSH
• vitamin B komplex MeSH