Mammarenaviruses are bi-segmented RNA viruses. They encompass viruses responsible for several severe diseases in humans. While performing a de novo assembly of a new virus found in a wild single-striped grass mouse in Tanzania, we found a single S but two divergent L segments. Natural co-infections, common within reptarenaviruses in captivity, were never reported for mammarenaviruses and never in a wild sample. This finding can have implications for virus evolution as co-infection could trigger viral recombination/reassortment in natural reservoirs.

• Klíčová slova
• Lemniscomys rosalia, Mafiga mammarenavirus, co-infection, recombination; BlobTools, zoonotic infections,
• Publikační typ
• časopisecké články MeSH

Trichuris spp. is a widespread nematode which parasitizes a wide range of mammalian hosts including rodents, the most diverse mammalian order. However, genetic data on rodent whipworms are still scarce, with only one published whole genome (Trichuris muris) despite an increasing demand for whole genome data. We sequenced the whipworm mitogenomes from seven rodent hosts belonging to three biogeographic regions (Palearctic, Afrotropical, and Indomalayan), including three previously described species: Trichuris cossoni, Trichurisarvicolae, and Trichurismastomysi. We assembled and annotated two complete and five almost complete mitogenomes (lacking only the long non-coding region) and performed comparative genomic and phylogenetic analyses. All the mitogenomes are circular, have the same organisation, and consist of 13 protein-coding, 2 rRNA, and 22 tRNA genes. The phylogenetic analysis supports geographical clustering of whipworm species and indicates that T. mastomysi found in Eastern Africa is able to infect multiple closely related rodent hosts. Our results are informative for species delimitation based on mitochondrial markers and could be further used in studies on phylogeny, phylogeography, and population genetics of rodent whipworms.

• Klíčová slova
• Afrotropical, Indomalayan, Trichuris, comparative genomics, mitogenomes, palearctic, phylogenetics, rodents, whipworms,
• Publikační typ
• časopisecké články MeSH

Rodents are a speciose group of mammals with strong zoonotic potential. Some parts of Africa are still underexplored for the occurrence of rodent-borne pathogens, despite this high potential. Angola is at the convergence of three major biogeographical regions of sub-Saharan Africa, each harbouring a specific rodent community. This rodent-rich area is, therefore, strategic for studying the diversity and evolution of rodent-borne viruses. In this study we examined 290 small mammals, almost all rodents, for the presence of mammarenavirus and hantavirus RNA. While no hantavirus was detected, we found three rodent species positive for distinct mammarenaviruses with a particularly high prevalence in Namaqua rock rats (Micaelamys namaquensis). We characterised four complete virus genomes, which showed typical mammarenavirus organisation. Phylogenetic and genetic distance analyses revealed: (i) the presence of a significantly divergent strain of Luna virus in Angolan representatives of the ubiquitous Natal multimammate mouse (Mastomys natalensis), (ii) a novel Okahandja-related virus associated with the Angolan lineage of Micaelamys namaquensis for which we propose the name Bitu virus (BITV) and (iii) the occurrence of a novel Mobala-like mammarenavirus in the grey-bellied pygmy mouse (Mus triton) for which we propose the name Kwanza virus (KWAV). This high virus diversity in a limited host sample size and in a relatively small geographical area supports the idea that Angola is a hotspot for mammarenavirus diversity.

• Klíčová slova
• Angola, Mastomys natalensis, Micaelamys namaquensis, Mus triton, hantaviruses, mammarenaviruses, phylogeny,
• MeSH
• Arenaviridae klasifikace   genetika   MeSH
• fylogeneze MeSH
• genom virový MeSH
• infekce viry z čeledi Arenaviridae veterinární   MeSH
• lékařská geografie MeSH
• prevalence MeSH
• RNA virová MeSH
• sekvenování celého genomu MeSH
• zdroje nemoci virologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• RNA virová MeSH