Herein, we present our findings of an early appearance of the Monkeypox virus in Prague, Czech Republic. A retrospective analysis of biological samples, carried out on the 28th of April, revealed a previously unrecognized case of Monkeypox virus (MPxV) infection. Subsequent data analysis confirmed that the virus strain belongs to the ongoing outbreak. Combined with clinical and epidemiological investigations, we extended the roots of the current outbreak at least back to 16th of April, 2022.

• Keywords
• infection, monkeypox virus, orthopoxvirus, outbreak, poxviridae, zoonosis,
• MeSH
• Disease Outbreaks MeSH
• Humans MeSH
• Mpox, Monkeypox * epidemiology   MeSH
• Retrospective Studies MeSH
• Monkeypox virus MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Czech Republic epidemiology   MeSH