Most cited article - PubMed ID 35616904
Precision Structural Interpretation of Site-Specific N-Glycans in Seminal Plasma
Selecting proper and efficient glycopeptide enrichment approaches are essential for mass spectrometry-based glycoproteomics since glycopeptides are usually with microheterogeneity and low abundance in most biological samples. Herein, we introduced a cotton hydrophilic interaction liquid chromatography (HILIC) approach for large-scale glycopeptide enrichment with 80% acetonitrile/1% trifluoroacetic acid as the optimal sample loading buffer. The comparison of cotton HILIC with Venusil HILIC and mixed anion-exchange (MAX) approaches indicated that cotton HILIC was superior in overall glycopeptide enrichment, whereas Venusil HILIC preferred in complex glycan structures and MAX performed better with high mannose glycans. Exploration of capacity and recovery rate of cotton HILIC illustrated that 5mg cotton packed in a 200μL tip achieved a reasonable glycopeptide enrichment performance (~6% recovery) from ~0.5mg peptides. In conclusion, cotton HILIC can be used as an optional glycopeptide enrichment approach in glycosylation analysis with its specific merit.
- Keywords
- Absorbent cotton, Enrichment, Glycopeptide, HILIC, MAX, Mass spectrometry,
- MeSH
- Chromatography, Liquid methods MeSH
- Glycopeptides * chemistry MeSH
- Glycosylation MeSH
- Hydrophobic and Hydrophilic Interactions MeSH
- Polysaccharides * MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Glycopeptides * MeSH
- Polysaccharides * MeSH