The transport of inorganic phosphate anions into yeast cells (after preincubation with glucose, fructose or another metabolizable sugar, and in the presence of glucose) shows two kinetic components with half-saturation constants of 40 mumol/L and 2.4 mmol/L. The uptake was strikingly stimulated by 2-deoxy-D-glucose (2-dGlc) at lower concentrations but inhibited above 100 mmol/L. A similar stimulation was caused by adenine (0.01-1 mmol/L) and a very small one by uracil and inorganic sulfate. It is suggested that either a phosphorylation reaction accompanies the transport (2-dGlc) or that some compounds stimulate the H(+)-ATPase more than inorganic phosphate itself and thus increase its rate of transport.

• MeSH
• adenin farmakologie   MeSH
• adenosintrifosfát metabolismus   MeSH
• deoxyglukosa farmakologie   MeSH
• fosfáty metabolismus   MeSH
• fosforylace MeSH
• iontový transport účinky léků   MeSH
• kinetika MeSH
• protonové ATPasy metabolismus   MeSH
• Saccharomyces cerevisiae účinky léků   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• adenin MeSH
• adenosintrifosfát MeSH
• deoxyglukosa MeSH
• fosfáty MeSH
• protonové ATPasy MeSH

Thermus flavus and T. ruber grew optimally at 75 and 60 degrees C, respectively, but transport of monosaccharides (D-quinovose) and amino acids (2-aminoisobutyric acid) had optima about 20 degree C lower. Both transports were active, inhibited by 2,4-dinitrophenol but hardly at all by uranyl(2+) ions. Several transport systems are apparently involved with each class of compounds. Preincubation with glucose curtailed subsequent transport severely. Practical cessation of transport below 35 degrees C may be associated with the rather uniform composition of membrane lipids where iso- and anteiso-C15 and C17 acids are practically the only components.

• MeSH
• aktivní transport MeSH
• aminokyseliny metabolismus   MeSH
• deoxyglukosa metabolismus   MeSH
• dinitrofenoly farmakologie   MeSH
• druhová specificita MeSH
• kyseliny aminoisomáselné metabolismus   MeSH
• mikrobiologie vody * MeSH
• monosacharidy metabolismus   MeSH
• Thermus růst a vývoj   metabolismus   MeSH
• vysoká teplota * MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• aminokyseliny MeSH
• deoxyglukosa MeSH
• dinitrofenoly MeSH
• kyseliny aminoisomáselné MeSH
• monosacharidy MeSH