The preparation of pure metabolites of bioactive compounds, particularly (poly)phenols, is essential for the accurate determination of their pharmacological profiles in vivo. Since the extraction of these metabolites from biological material is tedious and impractical, they can be synthesized enzymatically in vitro by bacterial PAPS-independent aryl sulfotransferases (ASTs). However, only a few ASTs have been studied and used for (poly)phenol sulfation. This study introduces new fully characterized recombinant ASTs selected according to their similarity to the previously characterized ASTs. These enzymes, produced in Escherichia coli, were purified, biochemically characterized, and screened for the sulfation of nine flavonoids and two phenolic acids using p-nitrophenyl sulfate. All tested compounds were proved to be substrates for the new ASTs, with kaempferol and luteolin being the best converted acceptors. ASTs from Desulfofalx alkaliphile (DalAST) and Campylobacter fetus (CfAST) showed the highest efficiency in the sulfation of tested polyphenols. To demonstrate the efficiency of the present sulfation approach, a series of new authentic metabolite standards, regioisomers of kaempferol sulfate, were enzymatically produced, isolated, and structurally characterized.

• Keywords
• aryl sulfotransferase, enzymatic sulfation, kaempferol sulfate, metabolite, polyphenol,
• MeSH
• Arylsulfotransferase * metabolism   chemistry   genetics   MeSH
• Bacterial Proteins metabolism   chemistry   genetics   MeSH
• Biocatalysis MeSH
• Escherichia coli metabolism   genetics   enzymology   MeSH
• Polyphenols * metabolism   chemistry   MeSH
• Sulfates metabolism   chemistry   MeSH
• Substrate Specificity MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Arylsulfotransferase * MeSH
• Bacterial Proteins MeSH
• Polyphenols * MeSH
• Sulfates MeSH

The term "biotransformation" refers to the process by which various compounds are biocatalyzed and enzymatically modified, as well as the metabolic changes that occur in organisms as a result of exposure to xenobiotics [...].

• MeSH
• Biological Products * MeSH
• Biotransformation MeSH
• Phytochemicals metabolism   MeSH
• Xenobiotics metabolism   MeSH
• Publication type
• Editorial MeSH
• Names of Substances
• Biological Products * MeSH
• Phytochemicals MeSH
• Xenobiotics MeSH