The aim of the study was to analyze the variables that modify the levels of oxidative DNA damage and lipid peroxidation in non-smoking mothers and their newborns from environmentally distinct localities of the Czech Republic: Ceske Budejovice (CB, an agricultural region) and Karvina (an industrial region). Personal, socio-economic and medical data, concentrations of particulate matter of aerodynamic diameter < 2.5 µm (PM2.5) and benzo[a]pyrene (B[a]P) in the ambient air, the activities of antioxidant mechanisms (superoxide dismutase, catalase, glutathione peroxidase) and antioxidant capacity), the levels of pro-inflammatory cytokines, the concentrations of persistent organic pollutants (POPs) in blood plasma/cord blood plasma and urinary levels of polycyclic aromatic hydrocarbons metabolites (OH-PAHs) were investigated as parameters potentially affecting the markers of DNA oxidation (8-oxo-7,8-dihydro-2'-deoxyguanosine, 8-oxodG) and lipid peroxidation (15-F2t-isoprostane, 15-F2t-IsoP). Significantly higher levels of POPs were detected in the plasma of mothers/newborns from CB (p < 0.001), while increased external levels of B[a]P and PM2.5, confirmed by analyzing urinary OH-PAHs, were found in Karvina subjects (p < 0.001). In mothers, multivariate analysis showed no significant difference in oxidative stress markers (15-F2t-IsoP, 8-oxodG) between the two localities. The analysis further revealed that neither in CB nor, unexpectedly, in Karvina, did PAH exposure affect maternal lipid peroxidation. Significant associations between OH-PAHs and 15-F2t-IsoP or 8-oxodG were observed only in newborns. In addition, multivariate analyses revealed a borderline significant association between locality and 8-oxodG in the urine of all newborns (p = 0.05). In conclusion, not only the maternal exposure of PAHs but also some POPs can negatively affect oxidative stress status in the early-life of newborns.

• Klíčová slova
• antioxidant response and inflammatory cytokines, environmental pollution, lipid peroxidation, maternal exposure to newborn, oxidative DNA damage,
• Publikační typ
• časopisecké články MeSH

Short- and medium-chain chlorinated paraffins (SCCPs and MCCPs) are environmental contaminants known for their persistence and bioaccumulation in fatty tissues. SCCPs are considered potential carcinogens and endocrine disruptors, with similar effects expected for MCCPs. This study investigated the body burden of SCCPs and MCCPs in residents of two regions of the Czech Republic with different levels of industrial pollution. Blood serum samples from 62 individuals in Ceske Budejovice (control area) and Ostrava (industrial area) were analysed. The results showed higher concentrations of SCCPs (<120-650 ng/g lipid weight (lw)) and MCCPs (<240-1530 ng/g lw) in Ostrava compared to Ceske Budejovice (SCCPs: <120-210 ng/g lw, MCCPs: <240-340 ng/g lw). The statistical analysis revealed no significant correlations between chemical concentrations and demographic variables such as age, BMI, or gender. The findings are consistent with European and Australian studies but significantly lower than levels reported in China. This is the first comprehensive survey of SCCPs and MCCPs in human blood serum in the Czech Republic and the second study in Europe. The data collected in this study are essential for assessing SCCPs and MCCPs. They will contribute to a better understanding the potential health risks associated with exposure to these chemicals.

• Klíčová slova
• chlorinated paraffins, endocrine disruptor, human biomonitoring,
• Publikační typ
• časopisecké články MeSH