Since cell dying in heart failure (HF) may vary based on the aetiology, we examined the main forms of regulated necrosis, such as necroptosis and pyroptosis, in the hearts damaged due to myocardial infarction (MI) or pressure overload. We also investigated the effects of a drug inhibiting RIP3, a proposed convergent point for both these necrosis-like cell death modes. In rat hearts, left ventricular function, remodelling, pro-cell death, and pro-inflammatory events were investigated, and the pharmacodynamic action of RIP3 inhibitor (GSK'872) was assessed. Regardless of the HF aetiology, the heart cells were dying due to necroptosis, albeit the upstream signals may be different. Pyroptosis was observed only in post-MI HF. The dysregulated miRNAs in post-MI hearts were accompanied by higher levels of a predicted target, HMGB1, its receptors (TLRs), as well as the exacerbation of inflammation likely originating from macrophages. The RIP3 inhibitor suppressed necroptosis, unlike pyroptosis, normalised the dysregulated miRNAs and tended to decrease collagen content and affect macrophage infiltration without affecting cardiac function or structure. The drug also mitigated the local heart inflammation and normalised the higher circulating HMGB1 in rats with post-MI HF. Elevated serum levels of HMGB1 were also detected in HF patients and positively correlated with C-reactive protein, highlighting pro-inflammatory axis. In conclusion, in MI-, but not pressure overload-induced HF, both necroptosis and pyroptosis operate and might underlie HF pathogenesis. The RIP3-targeting pharmacological intervention might protect the heart by preventing pro-death and pro-inflammatory mechanisms, however, additional strategies targeting multiple pro-death pathways may exhibit greater cardioprotection.

• Klíčová slova
• Heart failure, High mobility group box 1, Inflammation, Necroptosis, Pyroptosis, Receptor-interacting protein kinase 3,
• MeSH
• funkce levé komory srdeční účinky léků   MeSH
• inhibitory proteinkinas * farmakologie   MeSH
• kardiomyocyty * účinky léků   patologie   enzymologie   MeSH
• krysa rodu Rattus MeSH
• mikro RNA metabolismus   genetika   MeSH
• modely nemocí na zvířatech MeSH
• nekroptóza * účinky léků   MeSH
• nekróza MeSH
• potkani Sprague-Dawley MeSH
• pyroptóza * účinky léků   MeSH
• remodelace komor účinky léků   MeSH
• serin-threoninkinasy interagující s receptory * antagonisté a inhibitory   metabolismus   MeSH
• srdeční selhání * patologie   enzymologie   patofyziologie   farmakoterapie   etiologie   genetika   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• inhibitory proteinkinas * MeSH
• mikro RNA MeSH
• Ripk3 protein, rat MeSH Prohlížeč
• serin-threoninkinasy interagující s receptory * MeSH