Cells across biological kingdoms release extracellular vesicles (EVs) as a means of communication with other cells, be their friends or foes. This is indeed true for the intracellular malaria parasite Plasmodium falciparum (Pf), which utilizes EVs to transport bioactive molecules to various human host systems. Yet, the study of this mode of communication in malaria research is currently constrained due to limitations in high-resolution tools and the absence of commercial antibodies. Here, we demonstrate the power of an advanced spectral flow cytometry approach to robustly detect secreted EVs, isolated from Pf-infected red blood cells. By labeling both EV membrane lipids and the DNA cargo within (non-antibody staining approach), we were able to detect a subpopulation of parasitic-derived EVs enriched in DNA. Furthermore, we could quantitatively measure the DNA-carrying EVs isolated from two distinct blood stages of the parasite: rings and trophozoites. Our findings showcase the potential of spectral flow cytometry to monitor dynamic changes in nucleic acid cargo within pathogenic EVs.

• Klíčová slova
• DNA, cargo, extracellular vesicles, flow cytometry, host pathogen, malaria, parasite,
• MeSH
• erytrocyty * parazitologie   metabolismus   MeSH
• extracelulární vezikuly * metabolismus   MeSH
• lidé MeSH
• Plasmodium falciparum * metabolismus   genetika   MeSH
• protozoální DNA * metabolismus   MeSH
• průtoková cytometrie * metody   MeSH
• tropická malárie * parazitologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• protozoální DNA * MeSH