A Western blot method (WB) was adapted for rapid screening of antibodies against coxsackie virus B1-B6 in sera from patients with newly diagnosed insulin-dependent diabetes mellitus, myocarditis or febrile syndrome of suspected coxsackie viral aetiology. The use of a mixture of all 6 coxsackie virus B serotypes as the common antigen permitted a very rapid and inexpensive detection of antibody-positive sera for preliminary diagnosis and further detailed assay. Comparison of the results with those obtained in parallel run virus-neutralization tests showed a higher sensitivity and comparable specificity of WB.

• MeSH
• antigeny virové imunologie   MeSH
• coxsackie virózy krev   virologie   MeSH
• enterovirus B lidský imunologie   MeSH
• lidé MeSH
• neutralizační testy MeSH
• protilátky virové krev   MeSH
• senzitivita a specificita MeSH
• western blotting MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• antigeny virové MeSH
• protilátky virové MeSH