A comparative examination of reduced methyl [MV·](+) and benzyl [BV·](+) viologens (as artificial electron donors for quantitative estimation of the respiratory periplasmic (Nap) and membrane-embedded (Nar) nitrate reductases) using a newly constructed nap mutant strain of Paracocccus denitrificans was done. The activity with [MV·](+) was high in whole-cell assays, confirming that this compound donates electrons to Nar. Initial rates of the more lipophilic [BV·](+) were considerably lower, which was interpreted to be caused by an inhibition of the active transport of nitrate into the cells. Anionophoric activity of [BV·](+) was detectable but too low to effectively circumvent the inhibition of nitrate transporter.

• MeSH
• analýza jednotlivých buněk MeSH
• bakteriální proteiny chemie   genetika   metabolismus   MeSH
• benzylviologen metabolismus   MeSH
• dusičnany metabolismus   MeSH
• kinetika MeSH
• nitrátreduktasa chemie   genetika   metabolismus   MeSH
• oxidace-redukce MeSH
• Rhodobacteraceae chemie   enzymologie   genetika   MeSH
• transport elektronů MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Názvy látek
• bakteriální proteiny MeSH
• benzylviologen MeSH
• dusičnany MeSH
• nitrátreduktasa MeSH