Factor Ei isolated from Listeria monocytogenes caused in rabbits 1 d after intravenous administration activation of peripheral blood lymphocytes in terms of rRNA biosynthesis. Increase of the number of these active lymphocytes was observed not only in peripheral blood, but also in spleen, lung, kidney, and liver. when the number of lymphocytes was related to the amount of the organ tissue cells, the increment appeared significant only in the liver where the number of active lymphocytes exceeded the control value by one order of magnitude. Based upon this observation we concluded that the accumulation of Listeria factor Ei activated lymphocytes occurred in liver. This characteristic is considered an additional immunomodulative property of Listeria factor Ei similar to the Escherichia coli lipopolysaccharide.

• MeSH
• aktivace lymfocytů účinky léků   MeSH
• biologické faktory imunologie   farmakologie   MeSH
• játra imunologie   MeSH
• králíci MeSH
• ledviny imunologie   MeSH
• Listeria monocytogenes imunologie   MeSH
• počet lymfocytů účinky léků   MeSH
• podskupiny lymfocytů účinky léků   imunologie   MeSH
• slezina imunologie   MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické faktory MeSH
• Listeria monocytogenes factor Ei MeSH Prohlížeč

The avirulent strain Listeria monocytogenes (Welshimer) induced upon a single injection 4 weeks prior to challenge with Mycobacterium tuberculosis H37Rv resistance in guinea-pigs, which was manifested by a significant decrease of spleen weight and Feldman's index in immunized animals. The degree of resistance was dependent on the immunizing dose and time of administration. Repeated high doses of Listeria yielded only a low or no effect. Further increase of resistance was obtained using the BCG lipids; however, stimulation of resistance with listeria lipids was not successful and, finally, Freund's incomplete adjuvant was significantly effective in the Feldman index only. The BCG lipids, extracted with ethanol, which contained nitrogen and water-soluble substances, induced a significant resistance against M. tuberculosis infection. The chloroform-methanol BCG lipids were also effective, however, significantly less than the ethanol-extracted material. The listeria factor Ei itself or together with Freund's incomplete adjuvant possessed only low effectiveness against mycobacterial infection. However, if injected together with ethanol-extracted BCG lipids, it produced a significant degree of resistance which was higher than that induced by lipids only. The degree of resistance was comparable with the effect of living BCG strain which served as a source of isolated lipids.

• MeSH
• Freundovo adjuvans MeSH
• imunizace MeSH
• lipidy imunologie   MeSH
• Listeria monocytogenes imunologie   MeSH
• morčata MeSH
• Mycobacterium bovis imunologie   MeSH
• slezina MeSH
• tuberkulóza imunologie   MeSH
• velikost orgánu MeSH
• zvířata MeSH
• Check Tag
• morčata MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• Freundovo adjuvans MeSH
• lipidy MeSH