Factor Ei isolated from Listeria monocytogenes caused in rabbits 1 d after intravenous administration activation of peripheral blood lymphocytes in terms of rRNA biosynthesis. Increase of the number of these active lymphocytes was observed not only in peripheral blood, but also in spleen, lung, kidney, and liver. when the number of lymphocytes was related to the amount of the organ tissue cells, the increment appeared significant only in the liver where the number of active lymphocytes exceeded the control value by one order of magnitude. Based upon this observation we concluded that the accumulation of Listeria factor Ei activated lymphocytes occurred in liver. This characteristic is considered an additional immunomodulative property of Listeria factor Ei similar to the Escherichia coli lipopolysaccharide.

• MeSH
• Lymphocyte Activation drug effects   MeSH
• Biological Factors immunology   pharmacology   MeSH
• Liver immunology   MeSH
• Rabbits MeSH
• Kidney immunology   MeSH
• Listeria monocytogenes immunology   MeSH
• Lymphocyte Count drug effects   MeSH
• Lymphocyte Subsets drug effects   immunology   MeSH
• Spleen immunology   MeSH
• Animals MeSH
• Check Tag
• Rabbits MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Biological Factors MeSH
• Listeria monocytogenes factor Ei MeSH Browser

The avirulent strain Listeria monocytogenes (Welshimer) induced upon a single injection 4 weeks prior to challenge with Mycobacterium tuberculosis H37Rv resistance in guinea-pigs, which was manifested by a significant decrease of spleen weight and Feldman's index in immunized animals. The degree of resistance was dependent on the immunizing dose and time of administration. Repeated high doses of Listeria yielded only a low or no effect. Further increase of resistance was obtained using the BCG lipids; however, stimulation of resistance with listeria lipids was not successful and, finally, Freund's incomplete adjuvant was significantly effective in the Feldman index only. The BCG lipids, extracted with ethanol, which contained nitrogen and water-soluble substances, induced a significant resistance against M. tuberculosis infection. The chloroform-methanol BCG lipids were also effective, however, significantly less than the ethanol-extracted material. The listeria factor Ei itself or together with Freund's incomplete adjuvant possessed only low effectiveness against mycobacterial infection. However, if injected together with ethanol-extracted BCG lipids, it produced a significant degree of resistance which was higher than that induced by lipids only. The degree of resistance was comparable with the effect of living BCG strain which served as a source of isolated lipids.

• MeSH
• Freund's Adjuvant MeSH
• Immunization MeSH
• Lipids immunology   MeSH
• Listeria monocytogenes immunology   MeSH
• Guinea Pigs MeSH
• Mycobacterium bovis immunology   MeSH
• Spleen MeSH
• Tuberculosis immunology   MeSH
• Organ Size MeSH
• Animals MeSH
• Check Tag
• Guinea Pigs MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Freund's Adjuvant MeSH
• Lipids MeSH