A small-colony forming variant of Escherichia coli with a mutation in the ncf gene was analysed. The alternation of the protein composition in the cytoplasmic membrane and the interaction with K and E group colicins indicated a membrane mutation. The effect of this mutation on some membrane-bound processes, the activity of Mg2+-activated ATPase, the growth on different carbon sources and the active transport of amino acids, is described. This mutation does not exert any effect on the electron transport system.

• MeSH
• Adenosine Triphosphatases metabolism   MeSH
• Aerobiosis MeSH
• Amino Acids metabolism   MeSH
• Anaerobiosis MeSH
• Drug Resistance, Microbial MeSH
• Bacitracin pharmacology   MeSH
• Bacterial Proteins biosynthesis   MeSH
• Cell Membrane drug effects   metabolism   MeSH
• Sodium Dodecyl Sulfate pharmacology   MeSH
• Escherichia coli drug effects   growth & development   metabolism   MeSH
• Genes MeSH
• Glucose metabolism   MeSH
• Colicins pharmacology   MeSH
• Deoxycholic Acid pharmacology   MeSH
• Lactates metabolism   MeSH
• Membrane Proteins biosynthesis   MeSH
• Mutation * MeSH
• Vancomycin pharmacology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Adenosine Triphosphatases MeSH
• Amino Acids MeSH
• Bacitracin MeSH
• Bacterial Proteins MeSH
• Sodium Dodecyl Sulfate MeSH
• Glucose MeSH
• Colicins MeSH
• Deoxycholic Acid MeSH
• Lactates MeSH
• Membrane Proteins MeSH
• Vancomycin MeSH

• MeSH
• Drug Resistance, Microbial MeSH
• RNA, Bacterial biosynthesis   MeSH
• Chloramphenicol pharmacology   MeSH
• Dactinomycin pharmacology   MeSH
• DNA, Bacterial MeSH
• Escherichia coli drug effects   growth & development   MeSH
• Carbon Isotopes MeSH
• Conjugation, Genetic MeSH
• Mutation MeSH
• Neomycin pharmacology   MeSH
• Recombination, Genetic MeSH
• Salmonella typhimurium drug effects   growth & development   MeSH
• Uracil metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• RNA, Bacterial MeSH
• Chloramphenicol MeSH
• Dactinomycin MeSH
• DNA, Bacterial MeSH
• Carbon Isotopes MeSH
• Neomycin MeSH
• Uracil MeSH

• MeSH
• Adenosine Triphosphate metabolism   MeSH
• Cyclic AMP metabolism   MeSH
• Drug Resistance, Microbial * MeSH
• Bacterial Proteins biosynthesis   MeSH
• RNA, Bacterial biosynthesis   MeSH
• DNA, Bacterial MeSH
• Escherichia coli drug effects   growth & development   metabolism   MeSH
• Extrachromosomal Inheritance MeSH
• Carbon Isotopes MeSH
• Leucine metabolism   MeSH
• Mutation * MeSH
• Neomycin pharmacology   MeSH
• Uracil metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Adenosine Triphosphate MeSH
• Cyclic AMP MeSH
• Bacterial Proteins MeSH
• RNA, Bacterial MeSH
• DNA, Bacterial MeSH
• Carbon Isotopes MeSH
• Leucine MeSH
• Neomycin MeSH
• Uracil MeSH