Production of cellulases was followed in 4 cultures of higher fungi (Agrocybe cylindracea, Len tinus tigrinus, Pleurotus ostreatus, Ramaria formosa) cultivated on various substrates under different conditions. Stationary cultivation was more suitable than the submerged one. Addition of carboxymethy cellulose (CMC) was more suitable than addition of glucose. The cellulase activity in the presence of CMC was higher after a 12-d cultivation than after a 23-d period. Pine sawdust was most effective of all the substrates tested for the production of cellulases. Beech sawdust and wheat or rye straw were also useful. The addition of yeast autolyzate decreased the production of cellulases. A culture of L. tigrinud was the best producer.

• MeSH
• Basidiomycota enzymologie   MeSH
• celulasa biosyntéza   metabolismus   MeSH
• druhová specificita MeSH
• glukosa metabolismus   MeSH
• kultivační média * MeSH
• sodná sůl karboxymethylcelulosy metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• celulasa MeSH
• glukosa MeSH
• kultivační média * MeSH
• sodná sůl karboxymethylcelulosy MeSH

Nuclear ratios were studied in terminal and subterminal cells of various mycelia of the basidiomycete Oudemansiella mucida, the producer of the antifungal antibiotic mucidin (MuciderminR Spofa). The dikaryon, the monokaryon, and the mucidin-producing strain that had been cultivated for a long time under submerged conditions were compared. Dedikaryotization was found to have taken place in the producing strain. The originally dikaryotic culture with characteristic clamp connections on the mycelium and with two nuclei in every hyphal cell lost permanently the clamp connections, probably owing to continuous intense agitation. The hyphae contained solely mononuclear cells. Mating with a compatible monokaryon yielded a dikaryon capable of normal fructification.

• MeSH
• antifungální látky biosyntéza   MeSH
• bakteriologické techniky MeSH
• Basidiomycota cytologie   metabolismus   MeSH
• buněčné jádro MeSH
• kultivační média MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antifungální látky MeSH
• kultivační média MeSH