Intracellular proteolytic activities of B. megaterium KM occur soluble in the cytoplasm and periplasm and insoluble in the membrane. Two proteolytic enzymes were found in the cytoplasmic fraction by gel filtration on Sephadex G 150 and by polyacrylamide gel electrophoresis. The first enzyme called CI was stable, had a relative molecular mass of Mr = 105,000 (M = 105 kg/mol) and was inhibited by EDTA and PMSF, whereas the second, designated CII, was labile and had a relative molecular mass of Mr = 46,000 (M = 46 kg/mol). Because of its lability it could not be characterized in detail. In the "periplasm" only a single proteolytic enzyme P (Mr = 28,000; M = 28 kg/mol) inhibited by EDTA could be demonstrated. The extracellular enzyme exhibited similar properties. The membrane proteolytic activity was sensitive to PMSF and EDTA. The membrane enzymes have not yet been solubilized. In cells of the mutant KM 12 that does not produce the extracellular proteinase, only one type of proteinase, in all its properties identical with the cytoplasmic proteinase CI, could be demonstrated.

• MeSH
• Bacillus megaterium enzymologie   genetika   MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• endopeptidasy analýza   MeSH
• gelová chromatografie MeSH
• mutace MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• endopeptidasy MeSH

A strain of Pseudomonas aeruginosa from soil produced large quantities of extracellular neutral proteinase and could utilize several organic substances as carbon and nitrogen sources for enzyme production. The growth media required the presence of a high amount of phosphate when glucose was the carbon source. The intermediates of citric-acid cycle acids supported the proteinase production more than any other carbon sources. However, complex nitrogenous substances supported enzyme production more efficiently. Higher concentration of casamino acids suppressed the protinase synthesis.

• MeSH
• alkoholy farmakologie   MeSH
• aminokyseliny farmakologie   MeSH
• dusík farmakologie   MeSH
• endopeptidasy metabolismus   MeSH
• glukosa farmakologie   MeSH
• koncentrace vodíkových iontů MeSH
• kyseliny farmakologie   MeSH
• peptony farmakologie   MeSH
• Pseudomonas aeruginosa enzymologie   MeSH
• uhlík farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• alkoholy MeSH
• aminokyseliny MeSH
• casamino acids MeSH Prohlížeč
• dusík MeSH
• endopeptidasy MeSH
• glukosa MeSH
• kyseliny MeSH
• peptony MeSH
• uhlík MeSH

• MeSH
• autoradiografie MeSH
• Bacillus megaterium růst a vývoj   metabolismus   MeSH
• Bacillus subtilis enzymologie   MeSH
• bakteriální proteiny biosyntéza   metabolismus   MeSH
• bakteriologické techniky MeSH
• chlorid amonný metabolismus   MeSH
• dusík metabolismus   MeSH
• elektronová mikroskopie MeSH
• izotopy uhlíku MeSH
• kultivační média MeSH
• leucin metabolismus   MeSH
• proteasy biosyntéza   MeSH
• spory bakteriální růst a vývoj   MeSH
• tritium MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• bakteriální proteiny MeSH
• chlorid amonný MeSH
• dusík MeSH
• izotopy uhlíku MeSH
• kultivační média MeSH
• leucin MeSH
• proteasy MeSH
• tritium MeSH

• MeSH
• aminokyseliny farmakologie   MeSH
• Bacillus megaterium účinky léků   enzymologie   růst a vývoj   MeSH
• časové faktory MeSH
• cystein farmakologie   MeSH
• enzymová indukce MeSH
• enzymová represe MeSH
• glukosa farmakologie   MeSH
• izotopy uhlíku MeSH
• kultivační média MeSH
• peptony MeSH
• proteasy biosyntéza   MeSH
• spektrofotometrie MeSH
• spory růst a vývoj   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• aminokyseliny MeSH
• cystein MeSH
• glukosa MeSH
• izotopy uhlíku MeSH
• kultivační média MeSH
• peptony MeSH
• proteasy MeSH