The extracellular level of brefeldin A fluctuates with the length of malonate inhibition. Following treatment with malonate, mycelial multiplication as opposed to brefeldin A formation, was preferentially increased in the maleate, fumarate, succinate, citrate, methyl palmitate and glucose replacement cultures. Competitive maleate--malonate, fumarate--malonate, succinate--malonate and citrate--malonate-inhibited replacement cultures gave significantly higher mycelial and brefeldin A yields than the sole malonate-inhibited replacement cultures.

• MeSH
• antibakteriální látky biosyntéza   MeSH
• brefeldin A MeSH
• cyklopentany biosyntéza   MeSH
• kyseliny karboxylové metabolismus   MeSH
• malonáty farmakologie   MeSH
• mitosporické houby účinky léků   růst a vývoj   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• brefeldin A MeSH
• cyklopentany MeSH
• kyseliny karboxylové MeSH
• malonáty MeSH

Extracellular brefeldin A was detected in 4% glucose--peptone--mineral salts cultures of Curvularia lunata at the start of the exponential growth phase. Some fluctuations in brefeldin A levels occurred during the exponential growth phase followed by a significant reduction in level at the stationary growth phase. Broth glucose levels decreased according to a sigmoid relationship with time whereas broth pH remained fairly constant during the exponential growth phase followed by a gradual increase into the stationary growth phase. Mycelial brefeldin A levels were low throughout the various growth phases. The principal fatty acids present in decreasing order during the exponential growth phase were linoleic, oleic and palmitic acids. However, the content of linoleic acid was significantly reduced at the onset and during the stationary growth phase.

• MeSH
• antibakteriální látky biosyntéza   MeSH
• brefeldin A MeSH
• cyklopentany biosyntéza   MeSH
• glukosa metabolismus   MeSH
• koncentrace vodíkových iontů MeSH
• kultivační média MeSH
• kyseliny mastné neesterifikované metabolismus   MeSH
• mitosporické houby růst a vývoj   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• brefeldin A MeSH
• cyklopentany MeSH
• glukosa MeSH
• kultivační média MeSH
• kyseliny mastné neesterifikované MeSH

Malonate exerted a stronger inhibitory effect on brefeldin A production than on mycelia growth in cultures of Curvularia lunata especially at inhibitory levels of 100 to 200 mM. The extent of 200 mM malonate inhibition of growth and brefeldin A production was greater in cultures treated with malonate prior to inoculation than those treated following 5 days after inoculation. Maleate at levels of 40 to 220 mM activated brefeldin A formation in cultures though exerting variable effects on mycelia growth.

• MeSH
• antibakteriální látky biosyntéza   MeSH
• cyklopentany metabolismus   MeSH
• maleáty metabolismus   MeSH
• malonáty metabolismus   MeSH
• mitosporické houby růst a vývoj   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• antibakteriální látky MeSH
• cyklopentany MeSH
• maleáty MeSH
• malonáty MeSH