B. firmus activates human peripheral blood lymphocytes in vitro. Bacteria inactivated by heat or by formaldehyde were about equally effective, stimulating the blastic transformation of lymphocytes at doses of 10-200 mg/L and Ig formation in the culture at 10-500 mg/L. The action of formaldehyde treated B. firmus was compared with that of analogously inactivated B. subtilis, B. polymyxa, B. coagulans, B. megaterium, B. pumilus, B. cereus and B. lentus at a concentration of 100 mg/L. All these bacilli mildly stimulated blastic transformation and most of them substantially stimulated Ig formation, but B. firmus was the most efficient in stimulating the formation of Ig of all classes, in particular IgM and IgA. Its effect on Ig formation was comparable with that of PWM and was unusually high as compared with that of other bacteria. B. firmus is apparently a strong polyclonal activator of B lymphocytes. Its cells or their components could be potentially used for modulating immune reactions.

• MeSH
• aktivace lymfocytů * účinky léků   MeSH
• Bacillus účinky léků   fyziologie   MeSH
• formaldehyd farmakologie   MeSH
• imunoglobulinové izotypy biosyntéza   MeSH
• lidé MeSH
• lymfocyty imunologie   MeSH
• mitogeny líčidla amerického farmakologie   MeSH
• vysoká teplota MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• formaldehyd MeSH
• imunoglobulinové izotypy MeSH
• mitogeny líčidla amerického MeSH