Mumps vaccines are live attenuated viruses. They are known to vary in effectiveness, degree of attenuation and adverse event profile. However, the underlying reasons are poorly understood. We studied two closely related mumps vaccines which originate from the same attenuated Jeryl Lynn-5 strain but have different efficacies. Jeryl Lynn-Canine Kidney (JL-CK), produced on primary canine kidney cells, is less effective than RIT4385, which is produced on chicken embryo fibroblasts. JL-CK and RIT4385 could be distinguished by a number of in vitro and in vivo properties. JL-CK produced heterogeneous, generally smaller plaques than RIT4385, but gave 100-fold higher titres when grown in cells and showed a higher degree of hydrocephalus formation in neonatal rat brains. Sanger sequencing of JL-CK identified 14 regions of heterogeneity throughout the genome. Plaque purification of JL-CK demonstrated the presence of five different Jeryl Lynn-5 variants encompassing the 14 mutations. One JL-CK mutation was associated with a small plaque phenotype, the effects of the others in vitro or in vivo were less clear. Only 4% of the JL-CK population corresponded to the parental Jeryl Lynn-5 strain. Next generation sequencing of JL-CK and virus before and after growth in cell lines or neonatal rat brains showed that propagation in vitro or in vivo altered the population dramatically. Our findings indicate that growth of JL-CK in primary canine kidney cells resulted in the selection of a mixture of mumps virus variants that have different biological properties compared to the parent Jeryl Lynn-5 virus. We also report three previously unknown heterogenic regions within the N gene of the RIT4385 vaccine.

• Klíčová slova
• Deep sequencing, Jeryl Lynn, Mumps virus, Neurovirulence, Vaccines,
• MeSH
• epitelové buňky MeSH
• farmaceutická technologie metody   MeSH
• hydrocefalus patologie   virologie   MeSH
• krysa rodu Rattus MeSH
• kultivace virů metody   MeSH
• kultivované buňky MeSH
• kuřecí embryo MeSH
• modely nemocí na zvířatech MeSH
• novorozená zvířata MeSH
• plakové testy MeSH
• populační dynamika MeSH
• vakcína proti příušnicím aplikace a dávkování   imunologie   MeSH
• virová nálož MeSH
• virulence MeSH
• virus příušnic růst a vývoj   imunologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• kuřecí embryo MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• vakcína proti příušnicím MeSH