Naive and immune specific-pathogen-free rabbits were inoculated in the duodenum with sporocysts of Eimeria coecicola or Eimeria intestinalis. Samples were taken from the following tissues: duodenum (site of penetration of sporozoites), ileum (specific target site of the endogenous development of E. intestinalis), vermiform appendix (target site of E. coecicola) and two extraintestinal sites, mesenteric lymph nodes (MLNs), and spleen. The presence of sporozoites was checked by immunohistochemistry. In rabbits primary-infected with E. coecicola, large numbers of sporozoites were detected in the duodenum, extraintestinal sites, and vermiform appendix. The abundance of sporozoites in the spleen, MLN, and appendix was significantly reduced in the immune rabbits, and the migration seemed impeded. In the rabbits infected with E. intestinalis, sporozoites were absent in the spleen and MLN, indicating that the route of migration is different from that of E. coecicola. The number of sporozoites in the crypts of the ileum was markedly reduced in the immune animals.

• MeSH
• antigeny protozoální metabolismus   MeSH
• Eimeria růst a vývoj   imunologie   patogenita   MeSH
• interakce hostitele a parazita MeSH
• kokcidióza imunologie   parazitologie   MeSH
• králíci MeSH
• lymfatické uzliny parazitologie   MeSH
• organismy bez specifických patogenů MeSH
• parazitární nemoci střev * MeSH
• Peyerovy pláty metabolismus   parazitologie   MeSH
• protilátky protozoální imunologie   MeSH
• slezina parazitologie   MeSH
• sporozoiti růst a vývoj   imunologie   patologie   MeSH
• tenké střevo metabolismus   parazitologie   patologie   MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny protozoální MeSH
• protilátky protozoální MeSH

The endogenous cycle of Eimeria flavescens was studied in specific pathogen-free rabbits by means of histology and transmission electron microscopy. In total, five asexual generations were observed and two types of meronts and merozoites were found in each generation. Type A gave rise to a smaller number of thick polynucleate merozoites in which daughter merozoites were formed by endomerogony, while in the type B meronts slender uninucleate merozoites arose from ectomerogony. The first generation meronts were found in the crypts and proximal part of the villi of the duodenum and jejunum, whereas the three following generations developed in the superficial epithelium of the large intestine (cecum, vermiform appendix and colon). The last merogony as well as gamogony took place in crypts of the large intestine.

• MeSH
• Eimeria růst a vývoj   patogenita   ultrastruktura   MeSH
• elektronová mikroskopie MeSH
• kokcidióza parazitologie   patologie   MeSH
• králíci parazitologie   MeSH
• organismy bez specifických patogenů MeSH
• parazitární nemoci střev parazitologie   patologie   MeSH
• parazitární nemoci u zvířat parazitologie   patologie   MeSH
• stadia vývoje MeSH
• střevní sliznice parazitologie   patologie   MeSH
• zvířata MeSH
• Check Tag
• králíci parazitologie   MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH