Most cited article - PubMed ID 9162456
Predbezná identifikácia izolátov Borrelia burgdorferi z kliest'ov Ixodes ricinus východného Slovenska
[Preliminary identification of Borrelia burgdorferi isolated from Ixodes ricinus ticks in eastern Slovakia]
Ixodes ricinus ticks were collected by random collections from western and central Slovakia during the years 1996-98. The midgut content of 240 ticks was examined by dark-field microscopy and submitted for cultivation for the presence of borrelias. Spirochetes were found in 21 unfed and host-seeking adults and nymphs (8.8%). By the analysis of restriction fragment length polymorphism (RFLP) one sample from unfed I. ricinus male from western Slovakia was identified as a triple infection of Borrelia burgdorferi sensu stricto, B. garinii and B. afzelii. The simultaneous presence of different B. burgdorferi genospecies in one midgut sample (triple infection in the tick) could be observed only after the multipart amplification of denaturated DNA and subsequent pooling of the products for further analysis.
- MeSH
- Borrelia burgdorferi classification genetics isolation & purification MeSH
- Genotype MeSH
- Ixodes microbiology MeSH
- Polymerase Chain Reaction MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
