Biphenyl-utilizing polychlorinated biphenyls (PCB)-degrading bacteria were isolated from sites highly contaminated by PCBs, and their degradation abilities were determined using GC for typical commercial PCB mixtures (Delor 103 and Delor 106). Out of twelve strains which utilized biphenyl as a sole source of carbon and energy, strains Pseudomonas alcaligenes KP2 and P. fluorescens KP12, characterized by the BIOLOG identification system and the NEFERM test, were shown to significantly co-metabolize the PCB mixture Delor 103. DNA-DNA hybridization was used to compare both strains with well-known PCB-degraders Burkholderia cepacia strain LB400 and Ralstonia eutropha strain H850. The strain KP12 employs the same meta-fission route for degradation of chlorobenzoates as a chlorobiphenyl degrader Pseudomonas cepacia P166. Both isolates KP2 and KP12 belong to different phylogenetic groups, which indicates that the same geographical location does not ensure the same ancestor of degradative enzymes. We confirmed that also highly chlorinated and the most toxic congeners, which are contained in commercial PCB mixtures, can be biotransformed by members of indigenous bacterial-soil community under aerobic conditions.

• MeSH
• Biodegradation, Environmental MeSH
• Burkholderia cepacia isolation & purification   metabolism   MeSH
• Cupriavidus necator isolation & purification   metabolism   MeSH
• DNA, Bacterial chemistry   isolation & purification   MeSH
• Soil Pollutants metabolism   toxicity   MeSH
• Polychlorinated Biphenyls metabolism   MeSH
• Pseudomonas genetics   isolation & purification   metabolism   MeSH
• Soil Microbiology * MeSH
• Blotting, Southern MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Czech Republic MeSH
• Names of Substances
• DNA, Bacterial MeSH
• Soil Pollutants MeSH
• Polychlorinated Biphenyls MeSH

A strain of bacteria able to detoxicate the herbicide diuron in pure culture was isolated from sites contaminated with different urea herbicides. Diuron was used as a sole source of carbon and energy by this isolate which is a Gram-negative, aerobic, rod-shaped bacterium with a single polar flagellum, and grows at 40 degrees C. The strain has been identified as Pseudomonas sp.

• MeSH
• Biodegradation, Environmental MeSH
• Diuron metabolism   MeSH
• Herbicides metabolism   MeSH
• Culture Media MeSH
• Soil Pollutants metabolism   MeSH
• Pseudomonas growth & development   isolation & purification   metabolism   MeSH
• Soil Microbiology * MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Diuron MeSH
• Herbicides MeSH
• Culture Media MeSH
• Soil Pollutants MeSH