The cytopoxic effect of RL2 lactaptin (the recombinant analog of proteolytic fragment of human kappa-casein) toward tumor cells in vitro and in vivo presents it as a novel promising antitumor drug. The binding of any drug with serum proteins can affect their activity, distribution, rate of excretion and toxicity in the human body. Here, we studied the ability of RL2 to bind to various blood serum proteins. Using magnetic microparticles bearing by RL2 as an affinity matrix, in combination with mass spectrometry and western blot analysis, we found a number of blood serum proteins possessing affinity for RL2. Among them IgA, IgM and IgG subclasses of immunoglobulins, apolipoprotein A1 and various cortactin isoforms were identified. This data suggests that in the bloodstream RL2 lactaptin takes part in complicate protein-protein interactions, which can affect its activity.
- Klíčová slova
- RL2 antitumor agent, human blood serum proteins, identification, magnetic microparticles, mass spectrometry and western blotting, purification,
- MeSH
- chromatografie afinitní metody MeSH
- kaseiny metabolismus MeSH
- krevní proteiny analýza izolace a purifikace metabolismus MeSH
- kyseliny polymethakrylové chemie MeSH
- lidé MeSH
- magnety chemie MeSH
- mikrosféry MeSH
- protinádorové látky metabolismus MeSH
- rekombinantní proteiny metabolismus MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kaseiny MeSH
- krevní proteiny MeSH
- kyseliny polymethakrylové MeSH
- lactaptin, human MeSH Prohlížeč
- polyglycidyl methacrylate MeSH Prohlížeč
- protinádorové látky MeSH
- rekombinantní proteiny MeSH
